Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9342 | 5' | -50.2 | NC_002512.2 | + | 158797 | 0.66 | 0.999215 |
Target: 5'- cCGGGgcGUcgucgacuucucGGCCGCGAaccUGCGGGAGGu -3' miRNA: 3'- -GUCCauCAu-----------CUGGUGCU---ACGUCUUCU- -5' |
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9342 | 5' | -50.2 | NC_002512.2 | + | 191284 | 0.66 | 0.999079 |
Target: 5'- aCGGGga--GGGCgGCGggGCGGggGAc -3' miRNA: 3'- -GUCCaucaUCUGgUGCuaCGUCuuCU- -5' |
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9342 | 5' | -50.2 | NC_002512.2 | + | 166476 | 0.66 | 0.99888 |
Target: 5'- gCGGGgacGGcgGGACCGCGGguCGGAGGAa -3' miRNA: 3'- -GUCCa--UCa-UCUGGUGCUacGUCUUCU- -5' |
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9342 | 5' | -50.2 | NC_002512.2 | + | 121828 | 0.66 | 0.998744 |
Target: 5'- aCGGGgcgGGU-GACCugACGAucgcgguccgcaaccUGCGGAAGAa -3' miRNA: 3'- -GUCCa--UCAuCUGG--UGCU---------------ACGUCUUCU- -5' |
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9342 | 5' | -50.2 | NC_002512.2 | + | 97819 | 0.66 | 0.998646 |
Target: 5'- cCGGGgacgcggAGUAGAggGCGgcGCGGggGAa -3' miRNA: 3'- -GUCCa------UCAUCUggUGCuaCGUCuuCU- -5' |
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9342 | 5' | -50.2 | NC_002512.2 | + | 150043 | 0.66 | 0.998371 |
Target: 5'- gGGGgccggGGACgACGggGCGGGAGGg -3' miRNA: 3'- gUCCauca-UCUGgUGCuaCGUCUUCU- -5' |
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9342 | 5' | -50.2 | NC_002512.2 | + | 103273 | 0.66 | 0.998371 |
Target: 5'- -uGGU-GUAGACCACGuaggacccGCuGAGGAg -3' miRNA: 3'- guCCAuCAUCUGGUGCua------CGuCUUCU- -5' |
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9342 | 5' | -50.2 | NC_002512.2 | + | 149501 | 0.66 | 0.998249 |
Target: 5'- gCGGGUGGUcaaaAGGCCGCGGccggagcgggccgGcCAGGAGGa -3' miRNA: 3'- -GUCCAUCA----UCUGGUGCUa------------C-GUCUUCU- -5' |
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9342 | 5' | -50.2 | NC_002512.2 | + | 113826 | 0.67 | 0.99768 |
Target: 5'- gGGGUAGUAGGCgAUGAaGaCGG-AGAa -3' miRNA: 3'- gUCCAUCAUCUGgUGCUaC-GUCuUCU- -5' |
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9342 | 5' | -50.2 | NC_002512.2 | + | 88711 | 0.67 | 0.99768 |
Target: 5'- -cGGUAGcGGGCCAgGAagacgucGCGGAAGGu -3' miRNA: 3'- guCCAUCaUCUGGUgCUa------CGUCUUCU- -5' |
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9342 | 5' | -50.2 | NC_002512.2 | + | 157218 | 0.67 | 0.99676 |
Target: 5'- cCAGGUGcUGGACUAC--UGCAaGAAGAa -3' miRNA: 3'- -GUCCAUcAUCUGGUGcuACGU-CUUCU- -5' |
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9342 | 5' | -50.2 | NC_002512.2 | + | 119247 | 0.68 | 0.995556 |
Target: 5'- gAGGUGGU-GAUgACGuacGUGCGGAAGc -3' miRNA: 3'- gUCCAUCAuCUGgUGC---UACGUCUUCu -5' |
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9342 | 5' | -50.2 | NC_002512.2 | + | 206060 | 0.68 | 0.99483 |
Target: 5'- uCAGGUacagGGUGGccaccuggguGCCGCGGUGCAGcugcagguucGGGAg -3' miRNA: 3'- -GUCCA----UCAUC----------UGGUGCUACGUC----------UUCU- -5' |
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9342 | 5' | -50.2 | NC_002512.2 | + | 217731 | 0.68 | 0.994011 |
Target: 5'- aGGGgcGgcagGGACCGCGAcuUGaAGAAGAa -3' miRNA: 3'- gUCCauCa---UCUGGUGCU--ACgUCUUCU- -5' |
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9342 | 5' | -50.2 | NC_002512.2 | + | 103006 | 0.68 | 0.992993 |
Target: 5'- cCAGGUugauGUAGGCCGCGuaGUccaccacgggcucGUAGAAGAc -3' miRNA: 3'- -GUCCAu---CAUCUGGUGC--UA-------------CGUCUUCU- -5' |
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9342 | 5' | -50.2 | NC_002512.2 | + | 114196 | 0.69 | 0.984961 |
Target: 5'- uCGGGcccGgcGGCCAUGAggagGCGGAAGAu -3' miRNA: 3'- -GUCCau-CauCUGGUGCUa---CGUCUUCU- -5' |
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9342 | 5' | -50.2 | NC_002512.2 | + | 227477 | 0.7 | 0.981049 |
Target: 5'- gGGGgcGgccGGCCGCGAggagGUAGGAGAc -3' miRNA: 3'- gUCCauCau-CUGGUGCUa---CGUCUUCU- -5' |
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9342 | 5' | -50.2 | NC_002512.2 | + | 125196 | 0.7 | 0.981049 |
Target: 5'- cCAGGUGGccGuCCAgGAcGCGGAGGAg -3' miRNA: 3'- -GUCCAUCauCuGGUgCUaCGUCUUCU- -5' |
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9342 | 5' | -50.2 | NC_002512.2 | + | 228282 | 0.7 | 0.975404 |
Target: 5'- cCGGGUGGgagagggaggacgGGACC-CGggGCGGggGGa -3' miRNA: 3'- -GUCCAUCa------------UCUGGuGCuaCGUCuuCU- -5' |
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9342 | 5' | -50.2 | NC_002512.2 | + | 182028 | 0.71 | 0.971014 |
Target: 5'- -cGGUAGaAGGCCcucguGCGGUGCAGGAu- -3' miRNA: 3'- guCCAUCaUCUGG-----UGCUACGUCUUcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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