Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9343 | 5' | -52.8 | NC_002512.2 | + | 189985 | 0.66 | 0.993309 |
Target: 5'- -aCCGCGUCUaCAGGaaggucgaccucaagUUgGCCgCGGUCg -3' miRNA: 3'- caGGUGCAGAaGUCC---------------AAgUGG-GCUAG- -5' |
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9343 | 5' | -52.8 | NC_002512.2 | + | 176998 | 0.66 | 0.99293 |
Target: 5'- cGUCCAUGUaCUucgugggcgUCAGGgcCGCCUGcgCg -3' miRNA: 3'- -CAGGUGCA-GA---------AGUCCaaGUGGGCuaG- -5' |
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9343 | 5' | -52.8 | NC_002512.2 | + | 225700 | 0.66 | 0.990769 |
Target: 5'- cUCCgGCGUCcUCAGGaugugUCGCCCGc-- -3' miRNA: 3'- cAGG-UGCAGaAGUCCa----AGUGGGCuag -5' |
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9343 | 5' | -52.8 | NC_002512.2 | + | 200978 | 0.66 | 0.990769 |
Target: 5'- --aCACGUcCUUCAGGUcCACCUucUCg -3' miRNA: 3'- cagGUGCA-GAAGUCCAaGUGGGcuAG- -5' |
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9343 | 5' | -52.8 | NC_002512.2 | + | 71995 | 0.66 | 0.990405 |
Target: 5'- uUCCGCuUCUucaguacuuauuacUCAGGgcgUCucCCCGAUCa -3' miRNA: 3'- cAGGUGcAGA--------------AGUCCa--AGu-GGGCUAG- -5' |
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9343 | 5' | -52.8 | NC_002512.2 | + | 217167 | 0.66 | 0.98951 |
Target: 5'- cUCCAuCGUCgUCGGGUucaUCGCCCu--- -3' miRNA: 3'- cAGGU-GCAGaAGUCCA---AGUGGGcuag -5' |
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9343 | 5' | -52.8 | NC_002512.2 | + | 44051 | 0.66 | 0.98951 |
Target: 5'- gGUCCAC-UUUUCGGGg-CGCCUaGAUCg -3' miRNA: 3'- -CAGGUGcAGAAGUCCaaGUGGG-CUAG- -5' |
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9343 | 5' | -52.8 | NC_002512.2 | + | 209770 | 0.67 | 0.978932 |
Target: 5'- cUCCGCGUCUUUcGGcaaGCCCGGc- -3' miRNA: 3'- cAGGUGCAGAAGuCCaagUGGGCUag -5' |
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9343 | 5' | -52.8 | NC_002512.2 | + | 196609 | 0.67 | 0.978932 |
Target: 5'- cGUCCGCaccaUCUcCcGGUUCGCCCGGg- -3' miRNA: 3'- -CAGGUGc---AGAaGuCCAAGUGGGCUag -5' |
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9343 | 5' | -52.8 | NC_002512.2 | + | 193201 | 0.68 | 0.976591 |
Target: 5'- -aCCGCGUCUUCGGcucgccgUUGCCCGGc- -3' miRNA: 3'- caGGUGCAGAAGUCca-----AGUGGGCUag -5' |
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9343 | 5' | -52.8 | NC_002512.2 | + | 26260 | 0.68 | 0.971345 |
Target: 5'- -aCCGCGUUguaUCGaccggagauauGGUUCACgCCGGUCg -3' miRNA: 3'- caGGUGCAGa--AGU-----------CCAAGUG-GGCUAG- -5' |
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9343 | 5' | -52.8 | NC_002512.2 | + | 183111 | 0.68 | 0.971345 |
Target: 5'- gGUCUucgaGCGUCUgaucUAGGUUCaggGCCCGGUUc -3' miRNA: 3'- -CAGG----UGCAGAa---GUCCAAG---UGGGCUAG- -5' |
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9343 | 5' | -52.8 | NC_002512.2 | + | 152091 | 0.68 | 0.968427 |
Target: 5'- -cCCGCGUCccguUUCGGGUUUuuaUCGAUCa -3' miRNA: 3'- caGGUGCAG----AAGUCCAAGug-GGCUAG- -5' |
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9343 | 5' | -52.8 | NC_002512.2 | + | 169879 | 0.68 | 0.968427 |
Target: 5'- cGUCCGCagcuUCUUCAGcgcGUUCGCCUucuuGAUCg -3' miRNA: 3'- -CAGGUGc---AGAAGUC---CAAGUGGG----CUAG- -5' |
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9343 | 5' | -52.8 | NC_002512.2 | + | 223262 | 0.68 | 0.965304 |
Target: 5'- cGUCCGCGUCgUCGGGgggcgggaCGCCgCGcgCa -3' miRNA: 3'- -CAGGUGCAGaAGUCCaa------GUGG-GCuaG- -5' |
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9343 | 5' | -52.8 | NC_002512.2 | + | 219639 | 0.69 | 0.950653 |
Target: 5'- -cCCGCuUCUUCgagaaccggAGGUUCGCCCG-UCg -3' miRNA: 3'- caGGUGcAGAAG---------UCCAAGUGGGCuAG- -5' |
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9343 | 5' | -52.8 | NC_002512.2 | + | 216178 | 0.69 | 0.946429 |
Target: 5'- -cCCGCGUC-UCGGGaucgCGCCCguGAUCa -3' miRNA: 3'- caGGUGCAGaAGUCCaa--GUGGG--CUAG- -5' |
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9343 | 5' | -52.8 | NC_002512.2 | + | 191612 | 0.7 | 0.927725 |
Target: 5'- aGUCCucgGCGUCUUCucgcgcgaucccUCGCCCGAUCg -3' miRNA: 3'- -CAGG---UGCAGAAGucca--------AGUGGGCUAG- -5' |
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9343 | 5' | -52.8 | NC_002512.2 | + | 216872 | 0.73 | 0.831087 |
Target: 5'- cGUCCGCGUCgucgUUCAGGaUCGCCaGGUUc -3' miRNA: 3'- -CAGGUGCAG----AAGUCCaAGUGGgCUAG- -5' |
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9343 | 5' | -52.8 | NC_002512.2 | + | 176713 | 0.73 | 0.805373 |
Target: 5'- -aUCGCGUCcUCAGGUUCGCgUUGAUCu -3' miRNA: 3'- caGGUGCAGaAGUCCAAGUG-GGCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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