Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9347 | 3' | -55.2 | NC_002512.2 | + | 215489 | 0.66 | 0.979137 |
Target: 5'- uGGCGccuGCUcuacuUCGCCUCCGUGGUcGGu -3' miRNA: 3'- cCUGCucuCGG-----AGUGGAGGCACUA-CC- -5' |
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9347 | 3' | -55.2 | NC_002512.2 | + | 195636 | 0.66 | 0.979137 |
Target: 5'- gGGACGGGAcGaCCggCGgCUCCGUGGa-- -3' miRNA: 3'- -CCUGCUCU-C-GGa-GUgGAGGCACUacc -5' |
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9347 | 3' | -55.2 | NC_002512.2 | + | 150059 | 0.66 | 0.979137 |
Target: 5'- gGGGCGGGAgggggggaccGCCgcCGCCgCCGUcgggcGAUGGa -3' miRNA: 3'- -CCUGCUCU----------CGGa-GUGGaGGCA-----CUACC- -5' |
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9347 | 3' | -55.2 | NC_002512.2 | + | 137136 | 0.66 | 0.971866 |
Target: 5'- cGACGuaucgguGAGCCUgGCCUCCaUGAc-- -3' miRNA: 3'- cCUGCu------CUCGGAgUGGAGGcACUacc -5' |
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9347 | 3' | -55.2 | NC_002512.2 | + | 185968 | 0.66 | 0.971866 |
Target: 5'- uGGAgcuCGAGggcaacGGCCUguCCcugucgCCGUGGUGGg -3' miRNA: 3'- -CCU---GCUC------UCGGAguGGa-----GGCACUACC- -5' |
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9347 | 3' | -55.2 | NC_002512.2 | + | 220229 | 0.67 | 0.96908 |
Target: 5'- gGGACGAGugcGCC-CGCCUggCCGacAUGGu -3' miRNA: 3'- -CCUGCUCu--CGGaGUGGA--GGCacUACC- -5' |
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9347 | 3' | -55.2 | NC_002512.2 | + | 227433 | 0.67 | 0.966102 |
Target: 5'- cGGGCGcGGGCCgcggUCACCUUCGcgcUGccGGg -3' miRNA: 3'- -CCUGCuCUCGG----AGUGGAGGC---ACuaCC- -5' |
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9347 | 3' | -55.2 | NC_002512.2 | + | 195789 | 0.67 | 0.962928 |
Target: 5'- uGGACGuGGGCgUgUugCUCCGcUGcgGGg -3' miRNA: 3'- -CCUGCuCUCGgA-GugGAGGC-ACuaCC- -5' |
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9347 | 3' | -55.2 | NC_002512.2 | + | 214288 | 0.68 | 0.943957 |
Target: 5'- uGGccCGAGGGCUUCACCUUCGUcuucGGc -3' miRNA: 3'- -CCu-GCUCUCGGAGUGGAGGCAcua-CC- -5' |
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9347 | 3' | -55.2 | NC_002512.2 | + | 193091 | 0.68 | 0.939519 |
Target: 5'- cGGCGAGuGCCgcuUCAUCUCCGcGccGGa -3' miRNA: 3'- cCUGCUCuCGG---AGUGGAGGCaCuaCC- -5' |
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9347 | 3' | -55.2 | NC_002512.2 | + | 148444 | 0.69 | 0.919557 |
Target: 5'- aGGACGGGGGCCggGCCccCCGcggGAcGGa -3' miRNA: 3'- -CCUGCUCUCGGagUGGa-GGCa--CUaCC- -5' |
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9347 | 3' | -55.2 | NC_002512.2 | + | 121399 | 0.7 | 0.876197 |
Target: 5'- cGGACG-GAGCCgCGCCUggCGUGGaGGg -3' miRNA: 3'- -CCUGCuCUCGGaGUGGAg-GCACUaCC- -5' |
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9347 | 3' | -55.2 | NC_002512.2 | + | 113150 | 0.71 | 0.814719 |
Target: 5'- gGGGCGccGGCCUCGCCgcggCCGgGcgGGa -3' miRNA: 3'- -CCUGCucUCGGAGUGGa---GGCaCuaCC- -5' |
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9347 | 3' | -55.2 | NC_002512.2 | + | 145758 | 0.73 | 0.761988 |
Target: 5'- cGGACGAGGaCCUCACCgagacgCUGUGGccgcgGGa -3' miRNA: 3'- -CCUGCUCUcGGAGUGGa-----GGCACUa----CC- -5' |
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9347 | 3' | -55.2 | NC_002512.2 | + | 207748 | 0.73 | 0.761988 |
Target: 5'- gGGACGcuccgggguGGAGCC--GCCgCCGUGGUGGu -3' miRNA: 3'- -CCUGC---------UCUCGGagUGGaGGCACUACC- -5' |
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9347 | 3' | -55.2 | NC_002512.2 | + | 221340 | 0.73 | 0.752779 |
Target: 5'- uGGAucCGAGAccgcgGCCUCGCCgcUCCGUGucgGUGGu -3' miRNA: 3'- -CCU--GCUCU-----CGGAGUGG--AGGCAC---UACC- -5' |
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9347 | 3' | -55.2 | NC_002512.2 | + | 185243 | 0.74 | 0.706381 |
Target: 5'- uGGGCGAGAGCCUCgACCgcggucucuacucggUCGUcgccauGAUGGa -3' miRNA: 3'- -CCUGCUCUCGGAG-UGGa--------------GGCA------CUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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