Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9349 | 5' | -55.7 | NC_002512.2 | + | 205710 | 0.7 | 0.843693 |
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Target: 5'- aCCggCGACAGGcggcggcacaagUCGGggucGGGGggUAGg -3' miRNA: 3'- aGGuaGCUGUCC------------AGCCa---CCCCuuGUC- -5' |
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| 9349 | 5' | -55.7 | NC_002512.2 | + | 207716 | 0.68 | 0.899377 |
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Target: 5'- gCCAUCGcCAGcgccgaguccaucGUCGGcGGGGGACGc -3' miRNA: 3'- aGGUAGCuGUC-------------CAGCCaCCCCUUGUc -5' |
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| 9349 | 5' | -55.7 | NC_002512.2 | + | 209874 | 0.69 | 0.866312 |
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Target: 5'- -aCGcCGGCuacuacguGGUCGGUcgcGGGGAGCAGg -3' miRNA: 3'- agGUaGCUGu-------CCAGCCA---CCCCUUGUC- -5' |
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| 9349 | 5' | -55.7 | NC_002512.2 | + | 219835 | 0.66 | 0.958494 |
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Target: 5'- gUCgGUCGGgGGGUcCGGcccGGGGGCGGa -3' miRNA: 3'- -AGgUAGCUgUCCA-GCCac-CCCUUGUC- -5' |
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| 9349 | 5' | -55.7 | NC_002512.2 | + | 221076 | 0.66 | 0.968252 |
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Target: 5'- cUCCggCGGCGGaaCGGcgUGGGGGACGc -3' miRNA: 3'- -AGGuaGCUGUCcaGCC--ACCCCUUGUc -5' |
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| 9349 | 5' | -55.7 | NC_002512.2 | + | 221299 | 0.69 | 0.887154 |
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Target: 5'- -----aGGCcgGGGUCGGcGGGGAGCGGg -3' miRNA: 3'- agguagCUG--UCCAGCCaCCCCUUGUC- -5' |
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| 9349 | 5' | -55.7 | NC_002512.2 | + | 221587 | 0.81 | 0.30808 |
|
Target: 5'- cUCCGUCGACccggagggGGGUCGGUGgacggcgaggaGGGAGCGGg -3' miRNA: 3'- -AGGUAGCUG--------UCCAGCCAC-----------CCCUUGUC- -5' |
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| 9349 | 5' | -55.7 | NC_002512.2 | + | 227099 | 0.75 | 0.572301 |
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Target: 5'- gCCGcCGGcCGGGUCGGgcucagacgGGGGAGCGGg -3' miRNA: 3'- aGGUaGCU-GUCCAGCCa--------CCCCUUGUC- -5' |
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| 9349 | 5' | -55.7 | NC_002512.2 | + | 228405 | 0.7 | 0.843693 |
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Target: 5'- gUCCGgggGACGGGgCGcUGGGGGGCGGg -3' miRNA: 3'- -AGGUag-CUGUCCaGCcACCCCUUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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