Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9349 | 5' | -55.7 | NC_002512.2 | + | 149427 | 0.7 | 0.858963 |
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Target: 5'- aCCG-CGGCGGGggCGGgcgcgGGGGGAgAGg -3' miRNA: 3'- aGGUaGCUGUCCa-GCCa----CCCCUUgUC- -5' |
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| 9349 | 5' | -55.7 | NC_002512.2 | + | 205710 | 0.7 | 0.843693 |
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Target: 5'- aCCggCGACAGGcggcggcacaagUCGGggucGGGGggUAGg -3' miRNA: 3'- aGGuaGCUGUCC------------AGCCa---CCCCuuGUC- -5' |
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| 9349 | 5' | -55.7 | NC_002512.2 | + | 228405 | 0.7 | 0.843693 |
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Target: 5'- gUCCGgggGACGGGgCGcUGGGGGGCGGg -3' miRNA: 3'- -AGGUag-CUGUCCaGCcACCCCUUGUC- -5' |
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| 9349 | 5' | -55.7 | NC_002512.2 | + | 100057 | 0.72 | 0.752089 |
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Target: 5'- cUCCAUCGcgacgggggccGCGGGcCGGggacggagcggucaGGGGAACAGg -3' miRNA: 3'- -AGGUAGC-----------UGUCCaGCCa-------------CCCCUUGUC- -5' |
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| 9349 | 5' | -55.7 | NC_002512.2 | + | 160725 | 0.72 | 0.738947 |
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Target: 5'- -aCAUCGGgGGGgauuUCGG-GGGGAACGGc -3' miRNA: 3'- agGUAGCUgUCC----AGCCaCCCCUUGUC- -5' |
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| 9349 | 5' | -55.7 | NC_002512.2 | + | 153172 | 0.73 | 0.710241 |
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Target: 5'- aCCuacggggCGGCGGGcagCGGUGGGGAggGCGGc -3' miRNA: 3'- aGGua-----GCUGUCCa--GCCACCCCU--UGUC- -5' |
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| 9349 | 5' | -55.7 | NC_002512.2 | + | 189701 | 0.74 | 0.628568 |
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Target: 5'- gUCGUCGACguccgggggaugucGGGgCGGUGGGGAgGCAGg -3' miRNA: 3'- aGGUAGCUG--------------UCCaGCCACCCCU-UGUC- -5' |
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| 9349 | 5' | -55.7 | NC_002512.2 | + | 227099 | 0.75 | 0.572301 |
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Target: 5'- gCCGcCGGcCGGGUCGGgcucagacgGGGGAGCGGg -3' miRNA: 3'- aGGUaGCU-GUCCAGCCa--------CCCCUUGUC- -5' |
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| 9349 | 5' | -55.7 | NC_002512.2 | + | 221587 | 0.81 | 0.30808 |
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Target: 5'- cUCCGUCGACccggagggGGGUCGGUGgacggcgaggaGGGAGCGGg -3' miRNA: 3'- -AGGUAGCUG--------UCCAGCCAC-----------CCCUUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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