Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9352 | 3' | -56.3 | NC_002512.2 | + | 173328 | 0.66 | 0.945456 |
Target: 5'- -cCUGCGGGGU--CUUCGgUCccGGCa -3' miRNA: 3'- cuGAUGCCCCAcuGGAGCgAGuaCCG- -5' |
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9352 | 3' | -56.3 | NC_002512.2 | + | 117680 | 0.66 | 0.941063 |
Target: 5'- gGGCUACGGGucGACga-GCUgCGUGGCg -3' miRNA: 3'- -CUGAUGCCCcaCUGgagCGA-GUACCG- -5' |
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9352 | 3' | -56.3 | NC_002512.2 | + | 93486 | 0.66 | 0.941063 |
Target: 5'- uACUuCGGGGUaucgGACCUgucgGC-CAUGGCg -3' miRNA: 3'- cUGAuGCCCCA----CUGGAg---CGaGUACCG- -5' |
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9352 | 3' | -56.3 | NC_002512.2 | + | 148124 | 0.66 | 0.936447 |
Target: 5'- aGGCgGCGGuGGUGACCcacgaccaGCUCAUGa- -3' miRNA: 3'- -CUGaUGCC-CCACUGGag------CGAGUACcg -5' |
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9352 | 3' | -56.3 | NC_002512.2 | + | 105369 | 0.67 | 0.915738 |
Target: 5'- uGACgGCGGGGUucagacccaucGACCcggcgaCGCUCuugGGCg -3' miRNA: 3'- -CUGaUGCCCCA-----------CUGGa-----GCGAGua-CCG- -5' |
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9352 | 3' | -56.3 | NC_002512.2 | + | 182457 | 0.67 | 0.91 |
Target: 5'- --gUGCGGGcGUGG---UGCUCGUGGCa -3' miRNA: 3'- cugAUGCCC-CACUggaGCGAGUACCG- -5' |
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9352 | 3' | -56.3 | NC_002512.2 | + | 97485 | 0.67 | 0.91 |
Target: 5'- ---gACGGGGcacaugGugUUCGCggcCAUGGCg -3' miRNA: 3'- cugaUGCCCCa-----CugGAGCGa--GUACCG- -5' |
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9352 | 3' | -56.3 | NC_002512.2 | + | 207752 | 0.68 | 0.884847 |
Target: 5'- cGCUcCGGGGUGgaGCCgcCGC-CGUGGUg -3' miRNA: 3'- cUGAuGCCCCAC--UGGa-GCGaGUACCG- -5' |
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9352 | 3' | -56.3 | NC_002512.2 | + | 220790 | 0.68 | 0.884847 |
Target: 5'- -cCUGCuGGGGaucguccucGACC-CGCUCGUGGUc -3' miRNA: 3'- cuGAUG-CCCCa--------CUGGaGCGAGUACCG- -5' |
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9352 | 3' | -56.3 | NC_002512.2 | + | 220329 | 0.68 | 0.884847 |
Target: 5'- gGGCUcCGGGGggccggacGGCCUCGUcaacgcCGUGGCc -3' miRNA: 3'- -CUGAuGCCCCa-------CUGGAGCGa-----GUACCG- -5' |
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9352 | 3' | -56.3 | NC_002512.2 | + | 199600 | 0.68 | 0.878024 |
Target: 5'- cGAC-GCGGGG--GCCUC-CUCcAUGGCg -3' miRNA: 3'- -CUGaUGCCCCacUGGAGcGAG-UACCG- -5' |
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9352 | 3' | -56.3 | NC_002512.2 | + | 113147 | 0.69 | 0.863763 |
Target: 5'- ---cGCGGGGcgccGGCCUCGCcgcggCcgGGCg -3' miRNA: 3'- cugaUGCCCCa---CUGGAGCGa----GuaCCG- -5' |
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9352 | 3' | -56.3 | NC_002512.2 | + | 219984 | 0.7 | 0.790585 |
Target: 5'- aGACUGCGGG---ACCUCGCUCccccucgcccGGCg -3' miRNA: 3'- -CUGAUGCCCcacUGGAGCGAGua--------CCG- -5' |
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9352 | 3' | -56.3 | NC_002512.2 | + | 121325 | 0.7 | 0.781681 |
Target: 5'- aGAU--CGGcacGGUGACCUCGCUCc-GGCg -3' miRNA: 3'- -CUGauGCC---CCACUGGAGCGAGuaCCG- -5' |
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9352 | 3' | -56.3 | NC_002512.2 | + | 185509 | 0.72 | 0.706605 |
Target: 5'- gGGCUGCGcGGG-GGCCUgcCGCUCuucgaggggGUGGCc -3' miRNA: 3'- -CUGAUGC-CCCaCUGGA--GCGAG---------UACCG- -5' |
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9352 | 3' | -56.3 | NC_002512.2 | + | 44007 | 0.8 | 0.278703 |
Target: 5'- cACguugAgGGGGUGACCUUGCUCcgGGUg -3' miRNA: 3'- cUGa---UgCCCCACUGGAGCGAGuaCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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