Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9353 | 3' | -48 | NC_002512.2 | + | 166612 | 0.73 | 0.983318 |
Target: 5'- gGGGUCccaggg---CUCCGCGUcCGGGa -3' miRNA: 3'- -CCCAGauauauuuaGAGGCGCA-GCCC- -5' |
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9353 | 3' | -48 | NC_002512.2 | + | 220015 | 0.71 | 0.996154 |
Target: 5'- cGGGUCgagaggccgCUCCGUcUCGGGg -3' miRNA: 3'- -CCCAGauauauuuaGAGGCGcAGCCC- -5' |
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9353 | 3' | -48 | NC_002512.2 | + | 220164 | 0.7 | 0.998785 |
Target: 5'- gGGcGUCgug------CUCUGCGUCGGGa -3' miRNA: 3'- -CC-CAGauauauuuaGAGGCGCAGCCC- -5' |
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9353 | 3' | -48 | NC_002512.2 | + | 219037 | 0.69 | 0.999453 |
Target: 5'- uGGUCUAcGUc-GUCggCCGCGgcgCGGGg -3' miRNA: 3'- cCCAGAUaUAuuUAGa-GGCGCa--GCCC- -5' |
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9353 | 3' | -48 | NC_002512.2 | + | 196400 | 0.68 | 0.999766 |
Target: 5'- cGGGUCUGgcgcgggGAcgCggggggcggcggCCGgGUCGGGa -3' miRNA: 3'- -CCCAGAUaua----UUuaGa-----------GGCgCAGCCC- -5' |
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9353 | 3' | -48 | NC_002512.2 | + | 145513 | 0.68 | 0.999766 |
Target: 5'- cGGGgCUGcuccggcUCUgCGCGUCGGGc -3' miRNA: 3'- -CCCaGAUauauuu-AGAgGCGCAGCCC- -5' |
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9353 | 3' | -48 | NC_002512.2 | + | 27024 | 0.68 | 0.999776 |
Target: 5'- uGGUCUAUAaacGGAUCcaCCGCuGUCGGu -3' miRNA: 3'- cCCAGAUAUa--UUUAGa-GGCG-CAGCCc -5' |
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9353 | 3' | -48 | NC_002512.2 | + | 160493 | 0.68 | 0.999776 |
Target: 5'- uGGGUUcc-----AUCUCCGCGgcgcCGGGa -3' miRNA: 3'- -CCCAGauauauuUAGAGGCGCa---GCCC- -5' |
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9353 | 3' | -48 | NC_002512.2 | + | 38979 | 0.68 | 0.999824 |
Target: 5'- cGGGUCUGUGUAAAag--CGUGUCGuuuGGa -3' miRNA: 3'- -CCCAGAUAUAUUUagagGCGCAGC---CC- -5' |
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9353 | 3' | -48 | NC_002512.2 | + | 166046 | 0.67 | 0.999893 |
Target: 5'- gGGGUC-AUcaAGGUCuUCUGCG-CGGGc -3' miRNA: 3'- -CCCAGaUAuaUUUAG-AGGCGCaGCCC- -5' |
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9353 | 3' | -48 | NC_002512.2 | + | 111705 | 0.67 | 0.999893 |
Target: 5'- gGGGUCgcggAUGacgacgGAGUCUCCG-G-CGGGc -3' miRNA: 3'- -CCCAGa---UAUa-----UUUAGAGGCgCaGCCC- -5' |
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9353 | 3' | -48 | NC_002512.2 | + | 33432 | 0.67 | 0.999916 |
Target: 5'- uGGGUCgucGUGaucggagaacugcGAUCUCCG-GUCGuGGg -3' miRNA: 3'- -CCCAGauaUAU-------------UUAGAGGCgCAGC-CC- -5' |
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9353 | 3' | -48 | NC_002512.2 | + | 120671 | 0.67 | 0.999918 |
Target: 5'- cGGGUCgauaucucgGUGAGUUUCUGCuccgagCGGGc -3' miRNA: 3'- -CCCAGaua------UAUUUAGAGGCGca----GCCC- -5' |
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9353 | 3' | -48 | NC_002512.2 | + | 191723 | 0.67 | 0.999918 |
Target: 5'- --cUUUAaGUGGAcCUCCGCGUCGGu -3' miRNA: 3'- cccAGAUaUAUUUaGAGGCGCAGCCc -5' |
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9353 | 3' | -48 | NC_002512.2 | + | 205126 | 0.67 | 0.999937 |
Target: 5'- cGGGUCUuc----GUCUCCGa--CGGGa -3' miRNA: 3'- -CCCAGAuauauuUAGAGGCgcaGCCC- -5' |
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9353 | 3' | -48 | NC_002512.2 | + | 184046 | 0.67 | 0.999937 |
Target: 5'- uGGUCgaguucGUGUGAGa--CCGCGUCGGa -3' miRNA: 3'- cCCAGa-----UAUAUUUagaGGCGCAGCCc -5' |
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9353 | 3' | -48 | NC_002512.2 | + | 54445 | 0.66 | 0.999973 |
Target: 5'- cGGGUCU----GAAUCaCCGaCG-CGGGa -3' miRNA: 3'- -CCCAGAuauaUUUAGaGGC-GCaGCCC- -5' |
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9353 | 3' | -48 | NC_002512.2 | + | 54251 | 0.66 | 0.999973 |
Target: 5'- cGGGUCU----GAAUCaCCGaCG-CGGGa -3' miRNA: 3'- -CCCAGAuauaUUUAGaGGC-GCaGCCC- -5' |
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9353 | 3' | -48 | NC_002512.2 | + | 224112 | 0.66 | 0.999973 |
Target: 5'- cGGUCgugGUGAccAUCUcggccguggacCCGCGcCGGGa -3' miRNA: 3'- cCCAGauaUAUU--UAGA-----------GGCGCaGCCC- -5' |
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9353 | 3' | -48 | NC_002512.2 | + | 208893 | 0.66 | 0.99998 |
Target: 5'- gGGGcCgg---GAAUCcCCGCG-CGGGa -3' miRNA: 3'- -CCCaGauauaUUUAGaGGCGCaGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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