miRNA: 3'- cuaauaCACGAGCAGUGGCcUCCCaG- -5'
miRNA: 3'- cuaaUAC-ACGAGCa------GUGGCCU-CCCAG- -5'
miRNA: 3'- cuaaUACACGagCAGUGGC-CUCCCaG- -5'
miRNA: 3'- cuaauacaCGAGCaGUGGCCUCCCag -5'
miRNA: 3'- cuaauaCACG-------AGCAGUGGCCUCCCAG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9357 | 3' | -53.1 | NC_002512.2 | + | 26492 | 0.67 | 0.980096 |
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Target: 5'- -----cGUGCUCGUCAUUGaAGGGcCu -3' miRNA: 3'- cuaauaCACGAGCAGUGGCcUCCCaG- -5' |
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| 9357 | 3' | -53.1 | NC_002512.2 | + | 190136 | 0.69 | 0.952714 |
|
Target: 5'- ---cGUGcUGCUCGccuggccCACCGGGuGGGUCu -3' miRNA: 3'- cuaaUAC-ACGAGCa------GUGGCCU-CCCAG- -5' |
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| 9357 | 3' | -53.1 | NC_002512.2 | + | 157805 | 0.69 | 0.948597 |
|
Target: 5'- ---gGUGUGCagGUCGCUGcGGGGGcCg -3' miRNA: 3'- cuaaUACACGagCAGUGGC-CUCCCaG- -5' |
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| 9357 | 3' | -53.1 | NC_002512.2 | + | 149322 | 0.71 | 0.880892 |
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Target: 5'- -------gGCUCGcCGCCGGAGGGa- -3' miRNA: 3'- cuaauacaCGAGCaGUGGCCUCCCag -5' |
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| 9357 | 3' | -53.1 | NC_002512.2 | + | 165705 | 0.72 | 0.858069 |
|
Target: 5'- -----cGUGCgucugcuUCGUCGUCGGGGGGUCg -3' miRNA: 3'- cuaauaCACG-------AGCAGUGGCCUCCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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