Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9360 | 3' | -48.5 | NC_002512.2 | + | 190766 | 0.67 | 0.999795 |
Target: 5'- cUCUUCUAcguggaccgggucaACAGCcgcaUCCGCU--UCCUCg -3' miRNA: 3'- -AGAAGAU--------------UGUUG----AGGCGAgaAGGAGg -5' |
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9360 | 3' | -48.5 | NC_002512.2 | + | 198192 | 0.69 | 0.997307 |
Target: 5'- gUCggCgAACAGgUCCGCg--UCCUCCu -3' miRNA: 3'- -AGaaGaUUGUUgAGGCGagaAGGAGG- -5' |
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9360 | 3' | -48.5 | NC_002512.2 | + | 200584 | 0.69 | 0.998411 |
Target: 5'- --gUCcguCGugUCCuCUCUUCCUCCu -3' miRNA: 3'- agaAGauuGUugAGGcGAGAAGGAGG- -5' |
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9360 | 3' | -48.5 | NC_002512.2 | + | 214586 | 0.68 | 0.99891 |
Target: 5'- cCUg--GGCGACgagaCGCUCUUCCUgCa -3' miRNA: 3'- aGAagaUUGUUGag--GCGAGAAGGAgG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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