miRNA: 3'- -GGGGGGUUUaCAGAUCUAGC-Cuu--AGG- -5'
miRNA: 3'- gGGGGGUUUA-CagaUCUAGCCUuaGG- -5'
miRNA: 3'- gGGGGGUUUa-CAGAU--CuaGCCuuAGG- -5'
miRNA: 3'- gGGGGGuuuaCAGau----CUAGCCuuAGG- -5'
miRNA: 3'- -GGGGGgUUUACAG----AUCuaGcCUUAGG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9363 | 5' | -49.1 | NC_002512.2 | + | 177778 | 0.69 | 0.996411 |
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Target: 5'- gUCCUCCAGG-GUCUGGAUCGaGuacuUCUg -3' miRNA: 3'- -GGGGGGUUUaCAGAUCUAGC-Cuu--AGG- -5' |
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| 9363 | 5' | -49.1 | NC_002512.2 | + | 221895 | 0.72 | 0.984034 |
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Target: 5'- gCUCCCAGAUcGg--GGAUCGGGcgCCa -3' miRNA: 3'- gGGGGGUUUA-CagaUCUAGCCUuaGG- -5' |
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| 9363 | 5' | -49.1 | NC_002512.2 | + | 213539 | 0.72 | 0.979999 |
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Target: 5'- gCCCUCGGAgcGUCUGcuGcgCGGggUCCg -3' miRNA: 3'- gGGGGGUUUa-CAGAU--CuaGCCuuAGG- -5' |
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| 9363 | 5' | -49.1 | NC_002512.2 | + | 154398 | 0.72 | 0.975249 |
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Target: 5'- gCCCCC----GUCggaggcGAUCGGggUCCg -3' miRNA: 3'- gGGGGGuuuaCAGau----CUAGCCuuAGG- -5' |
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| 9363 | 5' | -49.1 | NC_002512.2 | + | 103191 | 0.75 | 0.934014 |
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Target: 5'- aCCCCCgGGGUGUCgacgUAGcgCaGGAUCCg -3' miRNA: 3'- -GGGGGgUUUACAG----AUCuaGcCUUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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