miRNA: 3'- aCUGGGGCGaGCAGuCGCCa------AAAGuc -5'
miRNA: 3'- aCUGGgGCG--AGCAGUCG-CCAaaa--GUC- -5'
miRNA: 3'- acUGGGGCG--------------AGCAGUCGCCaaaaguc -5'
miRNA: 3'- aCU-GGGGC-GAGCa----GUCGCCaaaaGUC- -5'
miRNA: 3'- -ACUGGgGCGAGcAGUCGCCaaaaGUc -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9365 | 5' | -54.6 | NC_002512.2 | + | 199626 | 0.66 | 0.976231 |
|
Target: 5'- cGGCUCCGCgCGUCcGCGGggacccgUUUCc- -3' miRNA: 3'- aCUGGGGCGaGCAGuCGCCa------AAAGuc -5' |
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| 9365 | 5' | -54.6 | NC_002512.2 | + | 202109 | 0.66 | 0.980733 |
|
Target: 5'- cGACCgCGUccUCGUCAGCuGGUggcgcCGGu -3' miRNA: 3'- aCUGGgGCG--AGCAGUCG-CCAaaa--GUC- -5' |
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| 9365 | 5' | -54.6 | NC_002512.2 | + | 213072 | 0.66 | 0.977886 |
|
Target: 5'- -aGCCCCGCgggcuacgugcucuUCGUCGGCGGc------ -3' miRNA: 3'- acUGGGGCG--------------AGCAGUCGCCaaaaguc -5' |
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| 9365 | 5' | -54.6 | NC_002512.2 | + | 218209 | 0.66 | 0.980733 |
|
Target: 5'- gGGcCCCCGcCUCGggccgCGGCGGgccgCGGc -3' miRNA: 3'- aCU-GGGGC-GAGCa----GUCGCCaaaaGUC- -5' |
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| 9365 | 5' | -54.6 | NC_002512.2 | + | 225050 | 0.67 | 0.950471 |
|
Target: 5'- aUGGCCgCGCUCcUCGGCGGcgcgCAa -3' miRNA: 3'- -ACUGGgGCGAGcAGUCGCCaaaaGUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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