Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9366 | 3' | -50.9 | NC_002512.2 | + | 126739 | 0.66 | 0.996836 |
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Target: 5'- cGGCgc-GU-GCCUC-UUGGCCCGAGa -3' miRNA: 3'- -CUGaauCAgUGGAGaAACCGGGCUUg -5' |
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| 9366 | 3' | -50.9 | NC_002512.2 | + | 214273 | 0.66 | 0.996836 |
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Target: 5'- cGCU--GUCGCCUggccUGGCCCGAGg -3' miRNA: 3'- cUGAauCAGUGGAgaa-ACCGGGCUUg -5' |
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| 9366 | 3' | -50.9 | NC_002512.2 | + | 50655 | 0.66 | 0.996285 |
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Target: 5'- aGCUUAGUCA-UUCUguauccUGGCaCCGAAUa -3' miRNA: 3'- cUGAAUCAGUgGAGAa-----ACCG-GGCUUG- -5' |
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| 9366 | 3' | -50.9 | NC_002512.2 | + | 171620 | 0.67 | 0.993237 |
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Target: 5'- -----cGUCGCCUUcg-GGCCCGAcaGCg -3' miRNA: 3'- cugaauCAGUGGAGaaaCCGGGCU--UG- -5' |
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| 9366 | 3' | -50.9 | NC_002512.2 | + | 156991 | 0.7 | 0.971476 |
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Target: 5'- aACUUcGGcCGCCgCUUcgGGCCCGAGCc -3' miRNA: 3'- cUGAA-UCaGUGGaGAAa-CCGGGCUUG- -5' |
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| 9366 | 3' | -50.9 | NC_002512.2 | + | 216306 | 0.7 | 0.968498 |
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Target: 5'- gGACgugGGUCACCugcaUCUgcGGCCCcGACg -3' miRNA: 3'- -CUGaa-UCAGUGG----AGAaaCCGGGcUUG- -5' |
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| 9366 | 3' | -50.9 | NC_002512.2 | + | 194308 | 0.72 | 0.926104 |
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Target: 5'- cGACa-GGUCACCUUccucgUGGCCCGGGa -3' miRNA: 3'- -CUGaaUCAGUGGAGaa---ACCGGGCUUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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