Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9366 | 5' | -55.2 | NC_002512.2 | + | 224139 | 0.66 | 0.950715 |
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Target: 5'- ---aCCCGcGCCGGGAGGCg------- -3' miRNA: 3'- uguaGGGC-CGGUCCUCCGaagauauu -5' |
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| 9366 | 5' | -55.2 | NC_002512.2 | + | 200398 | 0.66 | 0.950715 |
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Target: 5'- -gAUCUCGGCCGGGAGGg-------- -3' miRNA: 3'- ugUAGGGCCGGUCCUCCgaagauauu -5' |
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| 9366 | 5' | -55.2 | NC_002512.2 | + | 94284 | 0.67 | 0.937293 |
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Target: 5'- gGCGUCCaCGGCCGGGGaGCggUCg---- -3' miRNA: 3'- -UGUAGG-GCCGGUCCUcCGa-AGauauu -5' |
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| 9366 | 5' | -55.2 | NC_002512.2 | + | 88446 | 0.67 | 0.932347 |
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Target: 5'- gGCGUCCCGGaCCAGcAGGCg------- -3' miRNA: 3'- -UGUAGGGCC-GGUCcUCCGaagauauu -5' |
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| 9366 | 5' | -55.2 | NC_002512.2 | + | 226582 | 0.67 | 0.927163 |
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Target: 5'- ---cCCCGGCCAGGAGgGCg------- -3' miRNA: 3'- uguaGGGCCGGUCCUC-CGaagauauu -5' |
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| 9366 | 5' | -55.2 | NC_002512.2 | + | 152744 | 0.67 | 0.916078 |
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Target: 5'- gGCGUUCCGGCCGGGAGcCcggUCg---- -3' miRNA: 3'- -UGUAGGGCCGGUCCUCcGa--AGauauu -5' |
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| 9366 | 5' | -55.2 | NC_002512.2 | + | 220979 | 0.68 | 0.904044 |
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Target: 5'- gGCcgCCCuGGCCAGGAGGg-UCg---- -3' miRNA: 3'- -UGuaGGG-CCGGUCCUCCgaAGauauu -5' |
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| 9366 | 5' | -55.2 | NC_002512.2 | + | 127822 | 0.68 | 0.891076 |
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Target: 5'- -gGUCCCGGCUccGGGAGGUcgUCg---- -3' miRNA: 3'- ugUAGGGCCGG--UCCUCCGa-AGauauu -5' |
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| 9366 | 5' | -55.2 | NC_002512.2 | + | 208902 | 0.72 | 0.699643 |
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Target: 5'- -aAUCCCcGCgCGGGAGGCUcCUAUAAa -3' miRNA: 3'- ugUAGGGcCG-GUCCUCCGAaGAUAUU- -5' |
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| 9366 | 5' | -55.2 | NC_002512.2 | + | 181033 | 0.72 | 0.679457 |
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Target: 5'- cGCGUCCUGGgCAGGAGGCcgCa---- -3' miRNA: 3'- -UGUAGGGCCgGUCCUCCGaaGauauu -5' |
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| 9366 | 5' | -55.2 | NC_002512.2 | + | 153436 | 0.74 | 0.608024 |
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Target: 5'- gGCAggCCGGCCGGGcgcGGCUUUUAUAc -3' miRNA: 3'- -UGUagGGCCGGUCCu--CCGAAGAUAUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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