Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9367 | 5' | -47.5 | NC_002512.2 | + | 178556 | 0.66 | 0.999989 |
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Target: 5'- cCcgUCACGAUCCgGUugUgGugcgcguccGUCAGg -3' miRNA: 3'- aGuaAGUGCUAGGgUAugAgC---------UAGUC- -5' |
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| 9367 | 5' | -47.5 | NC_002512.2 | + | 152924 | 0.66 | 0.999962 |
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Target: 5'- gUCAgcgggaGCGGUCCCcgG-UCGGUCAGc -3' miRNA: 3'- -AGUaag---UGCUAGGGuaUgAGCUAGUC- -5' |
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| 9367 | 5' | -47.5 | NC_002512.2 | + | 159380 | 0.67 | 0.999913 |
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Target: 5'- cCAUUCGCgGGUCCCGcgGCgUCGGggCGGa -3' miRNA: 3'- aGUAAGUG-CUAGGGUa-UG-AGCUa-GUC- -5' |
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| 9367 | 5' | -47.5 | NC_002512.2 | + | 123432 | 0.67 | 0.999913 |
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Target: 5'- -uGUUCGCGAUCCCccgacagGCguUCGAUCc- -3' miRNA: 3'- agUAAGUGCUAGGGua-----UG--AGCUAGuc -5' |
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| 9367 | 5' | -47.5 | NC_002512.2 | + | 211522 | 0.68 | 0.99976 |
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Target: 5'- aUCGgcCGCGAUCUCAUGUUCGA-CAGc -3' miRNA: 3'- -AGUaaGUGCUAGGGUAUGAGCUaGUC- -5' |
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| 9367 | 5' | -47.5 | NC_002512.2 | + | 185254 | 0.69 | 0.999524 |
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Target: 5'- cUCGacCGCGGUCUC-UACUCGGUCGu -3' miRNA: 3'- -AGUaaGUGCUAGGGuAUGAGCUAGUc -5' |
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| 9367 | 5' | -47.5 | NC_002512.2 | + | 90013 | 0.69 | 0.998911 |
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Target: 5'- gCGgagCACGAUCCCGcACUCG-UCGa -3' miRNA: 3'- aGUaa-GUGCUAGGGUaUGAGCuAGUc -5' |
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| 9367 | 5' | -47.5 | NC_002512.2 | + | 141390 | 0.71 | 0.995602 |
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Target: 5'- gCGUcCGCGAUCCCGcACUCGGgaUCGa -3' miRNA: 3'- aGUAaGUGCUAGGGUaUGAGCU--AGUc -5' |
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| 9367 | 5' | -47.5 | NC_002512.2 | + | 15773 | 0.71 | 0.994874 |
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Target: 5'- gUAUcUACGAUCCCGauCUCGGUCAa -3' miRNA: 3'- aGUAaGUGCUAGGGUauGAGCUAGUc -5' |
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| 9367 | 5' | -47.5 | NC_002512.2 | + | 191625 | 0.72 | 0.994052 |
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Target: 5'- uUCucgCGCGAUCCCucGCcCGAUCGGa -3' miRNA: 3'- -AGuaaGUGCUAGGGuaUGaGCUAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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