Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9372 | 5' | -59 | NC_002512.2 | + | 90450 | 0.71 | 0.615481 |
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Target: 5'- aGGGCgGCGGcCGGGccagcuGCGGGUC-CCCGa -3' miRNA: 3'- -CCCG-UGCCaGUCC------UGCCUAGaGGGCa -5' |
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| 9372 | 5' | -59 | NC_002512.2 | + | 221397 | 0.73 | 0.511321 |
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Target: 5'- gGGGCGCGGgCGGGgaggcgGCGGGUCUgUCCGa -3' miRNA: 3'- -CCCGUGCCaGUCC------UGCCUAGA-GGGCa -5' |
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| 9372 | 5' | -59 | NC_002512.2 | + | 201865 | 0.74 | 0.492188 |
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Target: 5'- gGGGCcuGCGGUCcgAGGACGGcggcggcuucuccGUCUCCCc- -3' miRNA: 3'- -CCCG--UGCCAG--UCCUGCC-------------UAGAGGGca -5' |
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| 9372 | 5' | -59 | NC_002512.2 | + | 223010 | 0.75 | 0.406999 |
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Target: 5'- cGGCACGGcccaGGGACGGGUCgucgCCCa- -3' miRNA: 3'- cCCGUGCCag--UCCUGCCUAGa---GGGca -5' |
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| 9372 | 5' | -59 | NC_002512.2 | + | 225752 | 0.77 | 0.323262 |
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Target: 5'- gGGGCGCGGcCGGGACaugGGAUCcaucuguUCCCGa -3' miRNA: 3'- -CCCGUGCCaGUCCUG---CCUAG-------AGGGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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