Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9374 | 5' | -48 | NC_002512.2 | + | 198748 | 0.66 | 0.999967 |
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Target: 5'- gGUCcGCGaAGACGGcCUUCGUCUcgGg -3' miRNA: 3'- -UAGcUGCaUCUGUUaGAAGCAGGuaC- -5' |
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| 9374 | 5' | -48 | NC_002512.2 | + | 158712 | 0.66 | 0.999967 |
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Target: 5'- -cCGAgGUGGACGAcguguccuUCUUCGUCgAc- -3' miRNA: 3'- uaGCUgCAUCUGUU--------AGAAGCAGgUac -5' |
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| 9374 | 5' | -48 | NC_002512.2 | + | 179600 | 0.66 | 0.999941 |
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Target: 5'- cGUCGAUGUccGGGuccCGGUacUCGUCCAUGa -3' miRNA: 3'- -UAGCUGCA--UCU---GUUAgaAGCAGGUAC- -5' |
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| 9374 | 5' | -48 | NC_002512.2 | + | 180473 | 0.66 | 0.999941 |
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Target: 5'- cUCGGCGUcGcgucCAGUcCUUCGUCCGUc -3' miRNA: 3'- uAGCUGCAuCu---GUUA-GAAGCAGGUAc -5' |
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| 9374 | 5' | -48 | NC_002512.2 | + | 127589 | 0.66 | 0.999941 |
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Target: 5'- -gCGAUGUAGGCG----UCGUCCAc- -3' miRNA: 3'- uaGCUGCAUCUGUuagaAGCAGGUac -5' |
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| 9374 | 5' | -48 | NC_002512.2 | + | 122163 | 0.67 | 0.999785 |
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Target: 5'- uUCGACGUgaAGACGGUCU-CGgaucCCAa- -3' miRNA: 3'- uAGCUGCA--UCUGUUAGAaGCa---GGUac -5' |
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| 9374 | 5' | -48 | NC_002512.2 | + | 75358 | 0.68 | 0.999568 |
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Target: 5'- -gCGGCGgaccGGuaGCGAUCUUCGUCCu-- -3' miRNA: 3'- uaGCUGCa---UC--UGUUAGAAGCAGGuac -5' |
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| 9374 | 5' | -48 | NC_002512.2 | + | 33559 | 0.68 | 0.998999 |
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Target: 5'- -aCGACGUAGAuCAAUUUUCucggaucggauuGUCUAUGu -3' miRNA: 3'- uaGCUGCAUCU-GUUAGAAG------------CAGGUAC- -5' |
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| 9374 | 5' | -48 | NC_002512.2 | + | 219539 | 0.75 | 0.945615 |
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Target: 5'- cGUCGGCGUgcccaccGGACGGUCUUCG-CCGg- -3' miRNA: 3'- -UAGCUGCA-------UCUGUUAGAAGCaGGUac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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