Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9376 | 3' | -57.2 | NC_002512.2 | + | 128770 | 0.66 | 0.880511 |
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Target: 5'- uGCGcGAGCGCAcgccgcGCCGcGCGCUggCCGGg -3' miRNA: 3'- -UGUaCUCGCGU------UGGCcCGCGAa-GGUU- -5' |
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| 9376 | 3' | -57.2 | NC_002512.2 | + | 228132 | 0.66 | 0.880511 |
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Target: 5'- uGC-UGGGCGCcgaCGGGCGCgagcucaggUCCAc -3' miRNA: 3'- -UGuACUCGCGuugGCCCGCGa--------AGGUu -5' |
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| 9376 | 3' | -57.2 | NC_002512.2 | + | 192118 | 0.67 | 0.872732 |
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Target: 5'- cACggGAGCGaCAGCCGgcucagcuucuucGGCGCgggCCAGa -3' miRNA: 3'- -UGuaCUCGC-GUUGGC-------------CCGCGaa-GGUU- -5' |
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| 9376 | 3' | -57.2 | NC_002512.2 | + | 101795 | 0.67 | 0.866178 |
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Target: 5'- cGCGgcgGAGCGCuuCCGaGGagGCUUCCu- -3' miRNA: 3'- -UGUa--CUCGCGuuGGC-CCg-CGAAGGuu -5' |
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| 9376 | 3' | -57.2 | NC_002512.2 | + | 97762 | 0.74 | 0.489406 |
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Target: 5'- cGCAaccGGCGCuACCGGGCGCUgccggCCGAg -3' miRNA: 3'- -UGUac-UCGCGuUGGCCCGCGAa----GGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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