Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9377 | 5' | -49.4 | NC_002512.2 | + | 152697 | 0.66 | 0.999797 |
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Target: 5'- uCUCGUcgcGUCcCCGgUCGGggUGCGg- -3' miRNA: 3'- -GAGCAc--UAGaGGCaAGCCuuAUGCag -5' |
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| 9377 | 5' | -49.4 | NC_002512.2 | + | 41531 | 0.66 | 0.999797 |
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Target: 5'- aCUCG-GGUCUCgCGacgCGGAAguccgAUGUCu -3' miRNA: 3'- -GAGCaCUAGAG-GCaa-GCCUUa----UGCAG- -5' |
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| 9377 | 5' | -49.4 | NC_002512.2 | + | 181208 | 0.67 | 0.9995 |
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Target: 5'- -cCGUGAUCUCCGacacgUGGAccaccGCGUUg -3' miRNA: 3'- gaGCACUAGAGGCaa---GCCUua---UGCAG- -5' |
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| 9377 | 5' | -49.4 | NC_002512.2 | + | 123843 | 0.67 | 0.999383 |
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Target: 5'- -cCGUGAUCUgCGUggCGGAGaggACGcCg -3' miRNA: 3'- gaGCACUAGAgGCAa-GCCUUa--UGCaG- -5' |
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| 9377 | 5' | -49.4 | NC_002512.2 | + | 228706 | 0.67 | 0.999243 |
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Target: 5'- uCUCGUG--CUCCGUgucgCGGGGgaagACGUg -3' miRNA: 3'- -GAGCACuaGAGGCAa---GCCUUa---UGCAg -5' |
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| 9377 | 5' | -49.4 | NC_002512.2 | + | 43712 | 0.67 | 0.999077 |
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Target: 5'- aCUCGUucGUCUCCGauaUCGGAacGUACGa- -3' miRNA: 3'- -GAGCAc-UAGAGGCa--AGCCU--UAUGCag -5' |
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| 9377 | 5' | -49.4 | NC_002512.2 | + | 35684 | 0.76 | 0.869246 |
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Target: 5'- -cCGUGGUUUuuGUUCGGAGUA-GUCg -3' miRNA: 3'- gaGCACUAGAggCAAGCCUUAUgCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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