Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9378 | 5' | -53.4 | NC_002512.2 | + | 215345 | 0.66 | 0.99128 |
Target: 5'- -----cGGAUCGUgcCCGGCUCcGUCGAc -3' miRNA: 3'- caucuuCCUAGCA--GGCUGGGaCAGCU- -5' |
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9378 | 5' | -53.4 | NC_002512.2 | + | 220785 | 0.66 | 0.989963 |
Target: 5'- aUGGAccugcugGGGAUCGUCCucGACCCgcucgugGUCc- -3' miRNA: 3'- cAUCU-------UCCUAGCAGG--CUGGGa------CAGcu -5' |
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9378 | 5' | -53.4 | NC_002512.2 | + | 128656 | 0.66 | 0.989963 |
Target: 5'- --cGggGGAUCGagcUCCuccugcugcgggaGACCCUGgCGAa -3' miRNA: 3'- cauCuuCCUAGC---AGG-------------CUGGGACaGCU- -5' |
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9378 | 5' | -53.4 | NC_002512.2 | + | 225840 | 0.66 | 0.987329 |
Target: 5'- --cGggGGAUCGagCCGcUCCUGUCc- -3' miRNA: 3'- cauCuuCCUAGCa-GGCuGGGACAGcu -5' |
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9378 | 5' | -53.4 | NC_002512.2 | + | 94297 | 0.67 | 0.985743 |
Target: 5'- -gGGGAGcGGUCGUCC-ACCCgGUUGc -3' miRNA: 3'- caUCUUC-CUAGCAGGcUGGGaCAGCu -5' |
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9378 | 5' | -53.4 | NC_002512.2 | + | 59118 | 0.67 | 0.985743 |
Target: 5'- -gAGAAGGAccCGUCCGAUCCUca-GGa -3' miRNA: 3'- caUCUUCCUa-GCAGGCUGGGAcagCU- -5' |
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9378 | 5' | -53.4 | NC_002512.2 | + | 111494 | 0.67 | 0.98401 |
Target: 5'- aGUAGAcGGAgaguccggaCGUCCGccggcgguagcGCCCUGUCa- -3' miRNA: 3'- -CAUCUuCCUa--------GCAGGC-----------UGGGACAGcu -5' |
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9378 | 5' | -53.4 | NC_002512.2 | + | 99465 | 0.67 | 0.982122 |
Target: 5'- cGUAGAAGcGAcUGgCCGACaCCgUGUCGAu -3' miRNA: 3'- -CAUCUUC-CUaGCaGGCUG-GG-ACAGCU- -5' |
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9378 | 5' | -53.4 | NC_002512.2 | + | 211860 | 0.67 | 0.97545 |
Target: 5'- -gAGAAGGAccucagcaUCGUCgGcGCCCUGgagCGGg -3' miRNA: 3'- caUCUUCCU--------AGCAGgC-UGGGACa--GCU- -5' |
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9378 | 5' | -53.4 | NC_002512.2 | + | 218623 | 0.71 | 0.896212 |
Target: 5'- --cGAAGGAcgcgCGUCgGgACCCUGUCGc -3' miRNA: 3'- cauCUUCCUa---GCAGgC-UGGGACAGCu -5' |
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9378 | 5' | -53.4 | NC_002512.2 | + | 186001 | 0.72 | 0.868738 |
Target: 5'- cGUGGu-GGGUCGUCCGGCaCCUGg--- -3' miRNA: 3'- -CAUCuuCCUAGCAGGCUG-GGACagcu -5' |
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9378 | 5' | -53.4 | NC_002512.2 | + | 215594 | 0.72 | 0.837985 |
Target: 5'- cGUGGGAGGAcagcgUCGUCCGGgUCUG-CGGc -3' miRNA: 3'- -CAUCUUCCU-----AGCAGGCUgGGACaGCU- -5' |
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9378 | 5' | -53.4 | NC_002512.2 | + | 195065 | 0.74 | 0.767363 |
Target: 5'- -cGGGAGGGUCGUCCGgucggggACUCgagggGUCGAg -3' miRNA: 3'- caUCUUCCUAGCAGGC-------UGGGa----CAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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