Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9388 | 3' | -50.8 | NC_002512.2 | + | 176483 | 0.66 | 0.99891 |
Target: 5'- cCUGgAUGGCGgccGCCAGga-AGCCCGu -3' miRNA: 3'- aGACaUGUUGCa--UGGUCaagUCGGGC- -5' |
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9388 | 3' | -50.8 | NC_002512.2 | + | 107180 | 0.66 | 0.99891 |
Target: 5'- cCUGguugaagACGACGUGCaCGGgcacCAGCCgGg -3' miRNA: 3'- aGACa------UGUUGCAUG-GUCaa--GUCGGgC- -5' |
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9388 | 3' | -50.8 | NC_002512.2 | + | 213372 | 0.66 | 0.997753 |
Target: 5'- gCUGgcgGCGACaGUGCUAucgUCGGUCCGg -3' miRNA: 3'- aGACa--UGUUG-CAUGGUca-AGUCGGGC- -5' |
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9388 | 3' | -50.8 | NC_002512.2 | + | 164117 | 0.66 | 0.997753 |
Target: 5'- --aGUACAACGc-CCAGaagCAGUCCGu -3' miRNA: 3'- agaCAUGUUGCauGGUCaa-GUCGGGC- -5' |
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9388 | 3' | -50.8 | NC_002512.2 | + | 137128 | 0.67 | 0.996867 |
Target: 5'- aUCUacaACGACGUAUCGGUg-AGCCUGg -3' miRNA: 3'- -AGAca-UGUUGCAUGGUCAagUCGGGC- -5' |
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9388 | 3' | -50.8 | NC_002512.2 | + | 199876 | 0.67 | 0.996867 |
Target: 5'- uUCUGUGCGGCGgcucGCCcuGGUcUCGagaccGCCCGu -3' miRNA: 3'- -AGACAUGUUGCa---UGG--UCA-AGU-----CGGGC- -5' |
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9388 | 3' | -50.8 | NC_002512.2 | + | 207029 | 0.67 | 0.996326 |
Target: 5'- gUUGUGCAGCGUGCC-GUcCGGCa-- -3' miRNA: 3'- aGACAUGUUGCAUGGuCAaGUCGggc -5' |
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9388 | 3' | -50.8 | NC_002512.2 | + | 111480 | 0.67 | 0.995013 |
Target: 5'- gCUGUG-AGCGUGCCAGUagacggagAGUCCGg -3' miRNA: 3'- aGACAUgUUGCAUGGUCAag------UCGGGC- -5' |
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9388 | 3' | -50.8 | NC_002512.2 | + | 190349 | 0.68 | 0.991251 |
Target: 5'- cCUcUACGACG-ACgGGggcgUCGGCCCGg -3' miRNA: 3'- aGAcAUGUUGCaUGgUCa---AGUCGGGC- -5' |
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9388 | 3' | -50.8 | NC_002512.2 | + | 214749 | 0.68 | 0.990027 |
Target: 5'- cCUGcgcggaACAGCGUGCCGucgCAGUCCGg -3' miRNA: 3'- aGACa-----UGUUGCAUGGUcaaGUCGGGC- -5' |
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9388 | 3' | -50.8 | NC_002512.2 | + | 128198 | 0.7 | 0.97734 |
Target: 5'- --cGUACAGCGUgaggaaaccGCCGacGUagaUCGGCCCGg -3' miRNA: 3'- agaCAUGUUGCA---------UGGU--CA---AGUCGGGC- -5' |
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9388 | 3' | -50.8 | NC_002512.2 | + | 229690 | 0.73 | 0.904812 |
Target: 5'- ----cGCGACGggggGCCAGggCAGCCCa -3' miRNA: 3'- agacaUGUUGCa---UGGUCaaGUCGGGc -5' |
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9388 | 3' | -50.8 | NC_002512.2 | + | 287 | 0.73 | 0.904812 |
Target: 5'- ----cGCGACGggggGCCAGggCAGCCCa -3' miRNA: 3'- agacaUGUUGCa---UGGUCaaGUCGGGc -5' |
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9388 | 3' | -50.8 | NC_002512.2 | + | 219022 | 0.74 | 0.891793 |
Target: 5'- gCUGcUGCGACGUGCUGGUcuacgucgUCGGCCgCGg -3' miRNA: 3'- aGAC-AUGUUGCAUGGUCA--------AGUCGG-GC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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