Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9392 | 5' | -47.2 | NC_002513.1 | + | 15866 | 0.66 | 0.947859 |
Target: 5'- ---cAGCUCCAugaaugggggcGCCUUUAAcugGgGCAGCu -3' miRNA: 3'- cauuUUGAGGU-----------CGGAGAUU---UgCGUCG- -5' |
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9392 | 5' | -47.2 | NC_002513.1 | + | 16841 | 0.66 | 0.942571 |
Target: 5'- ---cAGCUgCAGCCUCUGGGacCGCucuGUg -3' miRNA: 3'- cauuUUGAgGUCGGAGAUUU--GCGu--CG- -5' |
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9392 | 5' | -47.2 | NC_002513.1 | + | 16446 | 0.66 | 0.936947 |
Target: 5'- uGUGAcGCgUCCA-CCU--AAGCGCAGCg -3' miRNA: 3'- -CAUUuUG-AGGUcGGAgaUUUGCGUCG- -5' |
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9392 | 5' | -47.2 | NC_002513.1 | + | 28858 | 0.66 | 0.930983 |
Target: 5'- aGUAAAugUCguGCUUC-GGACGCuuguGCc -3' miRNA: 3'- -CAUUUugAGguCGGAGaUUUGCGu---CG- -5' |
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9392 | 5' | -47.2 | NC_002513.1 | + | 7737 | 0.66 | 0.930983 |
Target: 5'- -gAAGGCcccuUCCAG-CUCUAAACG-AGCg -3' miRNA: 3'- caUUUUG----AGGUCgGAGAUUUGCgUCG- -5' |
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9392 | 5' | -47.2 | NC_002513.1 | + | 9761 | 0.67 | 0.924677 |
Target: 5'- uUGAccCUCaCGGUCUCUAAACacauuagaugGCGGCa -3' miRNA: 3'- cAUUuuGAG-GUCGGAGAUUUG----------CGUCG- -5' |
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9392 | 5' | -47.2 | NC_002513.1 | + | 4056 | 0.67 | 0.918031 |
Target: 5'- uGUGGAACUauaaaaaauaCAGUUUCUGGGgGCGGUg -3' miRNA: 3'- -CAUUUUGAg---------GUCGGAGAUUUgCGUCG- -5' |
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9392 | 5' | -47.2 | NC_002513.1 | + | 8449 | 0.69 | 0.852982 |
Target: 5'- -gGAAguGCcCCAGCCUCaaguaGCAGCa -3' miRNA: 3'- caUUU--UGaGGUCGGAGauuugCGUCG- -5' |
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9392 | 5' | -47.2 | NC_002513.1 | + | 547 | 0.69 | 0.823635 |
Target: 5'- -cAGAAuCUCCAGUCaaaagCUAAGCGCAaGUg -3' miRNA: 3'- caUUUU-GAGGUCGGa----GAUUUGCGU-CG- -5' |
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9392 | 5' | -47.2 | NC_002513.1 | + | 11319 | 0.69 | 0.823635 |
Target: 5'- -aAGAGCagCAGCCUaguaUUAGGCGuCAGCa -3' miRNA: 3'- caUUUUGagGUCGGA----GAUUUGC-GUCG- -5' |
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9392 | 5' | -47.2 | NC_002513.1 | + | 15657 | 0.7 | 0.792054 |
Target: 5'- -gAuuGCUgCGGCCa-UAGGCGCGGCg -3' miRNA: 3'- caUuuUGAgGUCGGagAUUUGCGUCG- -5' |
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9392 | 5' | -47.2 | NC_002513.1 | + | 15558 | 0.72 | 0.672108 |
Target: 5'- uUGGAACUCCAGUCaucgCgaaaaaggagacccUAAACGCAGUa -3' miRNA: 3'- cAUUUUGAGGUCGGa---G--------------AUUUGCGUCG- -5' |
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9392 | 5' | -47.2 | NC_002513.1 | + | 3054 | 0.77 | 0.418391 |
Target: 5'- uGgcGAACUCCAGCCcaguGACGCAGa -3' miRNA: 3'- -CauUUUGAGGUCGGagauUUGCGUCg -5' |
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9392 | 5' | -47.2 | NC_002513.1 | + | 18555 | 1.13 | 0.001838 |
Target: 5'- cGUAAAACUCCAGCCUCUAAACGCAGCc -3' miRNA: 3'- -CAUUUUGAGGUCGGAGAUUUGCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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