Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9402 | 3' | -45.5 | NC_002519.1 | + | 18674 | 0.68 | 0.96222 |
Target: 5'- aCUGCGuccauGUGUccuGUAAUGCcUGCGACu -3' miRNA: 3'- cGAUGUu----CACGuu-CAUUACG-ACGUUG- -5' |
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9402 | 3' | -45.5 | NC_002519.1 | + | 7658 | 0.69 | 0.918331 |
Target: 5'- uGCUACGAGUGCAAcUAAcGCaGgAGCc -3' miRNA: 3'- -CGAUGUUCACGUUcAUUaCGaCgUUG- -5' |
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9402 | 3' | -45.5 | NC_002519.1 | + | 38430 | 0.71 | 0.86206 |
Target: 5'- uUUGCAAGUGCGAGUGuUGCUa---- -3' miRNA: 3'- cGAUGUUCACGUUCAUuACGAcguug -5' |
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9402 | 3' | -45.5 | NC_002519.1 | + | 7346 | 0.71 | 0.86206 |
Target: 5'- uGCUGCAaccAGUGaaacaAAUGCUGCGGCc -3' miRNA: 3'- -CGAUGU---UCACguucaUUACGACGUUG- -5' |
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9402 | 3' | -45.5 | NC_002519.1 | + | 7195 | 0.72 | 0.791215 |
Target: 5'- uCUACAAGUGCGAccAAUGCgGCuACa -3' miRNA: 3'- cGAUGUUCACGUUcaUUACGaCGuUG- -5' |
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9402 | 3' | -45.5 | NC_002519.1 | + | 7241 | 0.78 | 0.492029 |
Target: 5'- aGCUACAAcucagGCAAccAAUGCUGCAACg -3' miRNA: 3'- -CGAUGUUca---CGUUcaUUACGACGUUG- -5' |
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9402 | 3' | -45.5 | NC_002519.1 | + | 7535 | 0.81 | 0.336066 |
Target: 5'- uGCUGCAguaAGUGaGGGUAAUGCUGCuACa -3' miRNA: 3'- -CGAUGU---UCACgUUCAUUACGACGuUG- -5' |
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9402 | 3' | -45.5 | NC_002519.1 | + | 7406 | 1.13 | 0.003379 |
Target: 5'- uGCUACAAGUGCAAGUAAUGCUGCAACc -3' miRNA: 3'- -CGAUGUUCACGUUCAUUACGACGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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