Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9402 | 5' | -58.7 | NC_002519.1 | + | 10243 | 0.66 | 0.366941 |
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Target: 5'- cAUGCacaCGCUUagUCAGGCUGCUGa- -3' miRNA: 3'- -UACGacgGCGAAg-AGUCCGACGACga -5' |
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| 9402 | 5' | -58.7 | NC_002519.1 | + | 4896 | 0.68 | 0.263412 |
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Target: 5'- gAUGCUGuuGCUcaccUUgGGGCUGCUuGCg -3' miRNA: 3'- -UACGACggCGAa---GAgUCCGACGA-CGa -5' |
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| 9402 | 5' | -58.7 | NC_002519.1 | + | 7574 | 0.68 | 0.245791 |
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Target: 5'- -aGCUacuuCgGCUUCUCAGGCggcuaccagugccucUGCUGCUg -3' miRNA: 3'- uaCGAc---GgCGAAGAGUCCG---------------ACGACGA- -5' |
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| 9402 | 5' | -58.7 | NC_002519.1 | + | 7705 | 0.75 | 0.087365 |
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Target: 5'- aAUGCUGaaGCUUCUgAGaCUGCUGCUg -3' miRNA: 3'- -UACGACggCGAAGAgUCcGACGACGA- -5' |
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| 9402 | 5' | -58.7 | NC_002519.1 | + | 7772 | 0.76 | 0.066851 |
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Target: 5'- -gGCUGCUGCUUUgguugcaCAGGCUGCgGCUg -3' miRNA: 3'- uaCGACGGCGAAGa------GUCCGACGaCGA- -5' |
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| 9402 | 5' | -58.7 | NC_002519.1 | + | 7369 | 1.06 | 0.000317 |
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Target: 5'- aAUGCUGCCGCUUCUCAGGCUGCUGCUg -3' miRNA: 3'- -UACGACGGCGAAGAGUCCGACGACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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