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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9403 | 5' | -49.3 | NC_002519.1 | + | 16778 | 0.66 | 0.864595 |
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Target: 5'- aGUGGGACGauccuuacAGUACCUGcccAGCGCu-- -3' miRNA: 3'- aUAUCCUGU--------UCGUGGACa--UCGUGuuc -5' |
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| 9403 | 5' | -49.3 | NC_002519.1 | + | 107 | 0.67 | 0.852856 |
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Target: 5'- --gGGGACAGGUACCUanuacangguuganGUGGCuuCAAa -3' miRNA: 3'- auaUCCUGUUCGUGGA--------------CAUCGu-GUUc -5' |
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| 9403 | 5' | -49.3 | NC_002519.1 | + | 26924 | 0.67 | 0.84634 |
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Target: 5'- --aAGGGCu-GCACCcaucUG-AGCACGAGg -3' miRNA: 3'- auaUCCUGuuCGUGG----ACaUCGUGUUC- -5' |
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| 9403 | 5' | -49.3 | NC_002519.1 | + | 25622 | 0.67 | 0.836806 |
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Target: 5'- aGUGGGACuucccucuGGCAgUCUGUAGUagACGGGg -3' miRNA: 3'- aUAUCCUGu-------UCGU-GGACAUCG--UGUUC- -5' |
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| 9403 | 5' | -49.3 | NC_002519.1 | + | 22201 | 1.07 | 0.002901 |
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Target: 5'- gUAUAGGACAAGCACCUGUAGCACAAGc -3' miRNA: 3'- -AUAUCCUGUUCGUGGACAUCGUGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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