Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9405 | 3' | -44.8 | NC_002519.1 | + | 30621 | 0.66 | 0.989834 |
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Target: 5'- aGAGGCUACCuc--AGCugcucgugAACUUAACa -3' miRNA: 3'- aUUCCGAUGGcaauUCG--------UUGAAUUGa -5' |
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| 9405 | 3' | -44.8 | NC_002519.1 | + | 34502 | 0.67 | 0.986288 |
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Target: 5'- -cAGGUUACUGUUGAGUA---UGACa -3' miRNA: 3'- auUCCGAUGGCAAUUCGUugaAUUGa -5' |
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| 9405 | 3' | -44.8 | NC_002519.1 | + | 31851 | 0.67 | 0.984192 |
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Target: 5'- gAGGGUcuUGCagaGUUAAGUuGCUUAACg -3' miRNA: 3'- aUUCCG--AUGg--CAAUUCGuUGAAUUGa -5' |
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| 9405 | 3' | -44.8 | NC_002519.1 | + | 1423 | 0.72 | 0.870333 |
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Target: 5'- uUGAGGUUGCCGUUAAGCcuAgUUcAGCa -3' miRNA: 3'- -AUUCCGAUGGCAAUUCGu-UgAA-UUGa -5' |
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| 9405 | 3' | -44.8 | NC_002519.1 | + | 27556 | 0.72 | 0.832208 |
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Target: 5'- gGGGGCUACUGcuaaagcUAAGCAACUUAu-- -3' miRNA: 3'- aUUCCGAUGGCa------AUUCGUUGAAUuga -5' |
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| 9405 | 3' | -44.8 | NC_002519.1 | + | 31928 | 0.96 | 0.047229 |
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Target: 5'- gUAAGGCUACCGUU-AGCAACUUAACUc -3' miRNA: 3'- -AUUCCGAUGGCAAuUCGUUGAAUUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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