Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9407 | 3' | -50 | NC_002519.1 | + | 29115 | 0.66 | 0.876587 |
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Target: 5'- -gAGCAGCUguCaUUUUAGGCG-AGAa -3' miRNA: 3'- caUCGUCGAguGgAAAGUCCGUuUCU- -5' |
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| 9407 | 3' | -50 | NC_002519.1 | + | 25599 | 0.66 | 0.862807 |
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Target: 5'- uGUAGUagacggggcugcagaGGCUCugGCC--UCAGGUAAGGAa -3' miRNA: 3'- -CAUCG---------------UCGAG--UGGaaAGUCCGUUUCU- -5' |
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| 9407 | 3' | -50 | NC_002519.1 | + | 37751 | 0.68 | 0.779633 |
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Target: 5'- -cAGCAGCuUCugUcUUCAGGUAGuuAGAa -3' miRNA: 3'- caUCGUCG-AGugGaAAGUCCGUU--UCU- -5' |
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| 9407 | 3' | -50 | NC_002519.1 | + | 24772 | 0.68 | 0.735088 |
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Target: 5'- gGUAGguGacaUCACCa---AGGCAGAGAc -3' miRNA: 3'- -CAUCguCg--AGUGGaaagUCCGUUUCU- -5' |
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| 9407 | 3' | -50 | NC_002519.1 | + | 26544 | 0.75 | 0.384791 |
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Target: 5'- uUAGCAGCUguauccuugaaACCUUUCAGGCGcuAGGGg -3' miRNA: 3'- cAUCGUCGAg----------UGGAAAGUCCGU--UUCU- -5' |
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| 9407 | 3' | -50 | NC_002519.1 | + | 3142 | 1.1 | 0.001506 |
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Target: 5'- aGUAGCAGCUCACCUUUCAGGCAAAGAg -3' miRNA: 3'- -CAUCGUCGAGUGGAAAGUCCGUUUCU- -5' |
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| 9407 | 3' | -50 | NC_002519.1 | + | 39743 | 1.1 | 0.001506 |
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Target: 5'- aGUAGCAGCUCACCUUUCAGGCAAAGAg -3' miRNA: 3'- -CAUCGUCGAGUGGAAAGUCCGUUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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