miRNA: 3'- -UUCGUCGGUuCCGUcgUAGaaAUCUU- -5'
miRNA: 3'- -UUCGUCGGUUCCGUCGuAGAAAUcuu -5'
miRNA: 3'- -UUCGUCGGUUCCGUCGUAGAAAUCUU- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9409 | 3' | -51.6 | NC_002519.1 | + | 6760 | 0.69 | 0.522864 |
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Target: 5'- uGAGCAGCCGuGGCGuaAUCagUAGAGg -3' miRNA: 3'- -UUCGUCGGUuCCGUcgUAGaaAUCUU- -5' |
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| 9409 | 3' | -51.6 | NC_002519.1 | + | 30562 | 0.75 | 0.234401 |
|
Target: 5'- cGAGCAGCUGAGGUAGCcUCUUUGc-- -3' miRNA: 3'- -UUCGUCGGUUCCGUCGuAGAAAUcuu -5' |
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| 9409 | 3' | -51.6 | NC_002519.1 | + | 7041 | 1.04 | 0.001927 |
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Target: 5'- aAAGCAGCCAAGGCAGCAUCUUUAGAAg -3' miRNA: 3'- -UUCGUCGGUUCCGUCGUAGAAAUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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