Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9410 | 3' | -46 | NC_002519.1 | + | 17835 | 0.68 | 0.956064 |
Target: 5'- cAAGAAAGGaGAUGAGuuGGAGggGCUg -3' miRNA: 3'- aUUCUUUCCcCUAUUU--CCUCaaUGGa -5' |
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9410 | 3' | -46 | NC_002519.1 | + | 31949 | 0.69 | 0.922681 |
Target: 5'- ---cGAAGGGGAUAgcugaAGGGGGUaaggcUACCg -3' miRNA: 3'- auucUUUCCCCUAU-----UUCCUCA-----AUGGa -5' |
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9410 | 3' | -46 | NC_002519.1 | + | 8787 | 0.84 | 0.234194 |
Target: 5'- gGAGGAAGGGGGgauAGGGGGUgGCCUg -3' miRNA: 3'- aUUCUUUCCCCUau-UUCCUCAaUGGA- -5' |
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9410 | 3' | -46 | NC_002519.1 | + | 8477 | 1.08 | 0.00621 |
Target: 5'- aUAAGAAAGGGGAUAAAGGAGUUACCUg -3' miRNA: 3'- -AUUCUUUCCCCUAUUUCCUCAAUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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