Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9411 | 3' | -44.7 | NC_002519.1 | + | 23752 | 0.68 | 0.970892 |
Target: 5'- --gGUGGGCuacugcucGACAGAGGCCUCCa-- -3' miRNA: 3'- guaUAUCUGu-------UUGUUUUCGGGGGaac -5' |
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9411 | 3' | -44.7 | NC_002519.1 | + | 30603 | 0.7 | 0.94979 |
Target: 5'- ----gAGACu-GCGGAAGCCuCCCUUa -3' miRNA: 3'- guauaUCUGuuUGUUUUCGG-GGGAAc -5' |
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9411 | 3' | -44.7 | NC_002519.1 | + | 27497 | 0.7 | 0.944592 |
Target: 5'- ---uUAG-CuuuAGCAGuAGCCCCCUUGg -3' miRNA: 3'- guauAUCuGu--UUGUUuUCGGGGGAAC- -5' |
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9411 | 3' | -44.7 | NC_002519.1 | + | 23838 | 1.12 | 0.005506 |
Target: 5'- aCAUAUAGACAAACAAAAGCCCCCUUGg -3' miRNA: 3'- -GUAUAUCUGUUUGUUUUCGGGGGAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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