Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9413 | 5' | -51.4 | NC_002531.1 | + | 19486 | 0.66 | 0.981454 |
Target: 5'- --uAGugAGUCAUACaCCAGGaaGUCa -3' miRNA: 3'- auuUUugUCAGUGUG-GGUCCgcCAGa -5' |
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9413 | 5' | -51.4 | NC_002531.1 | + | 127783 | 0.66 | 0.97671 |
Target: 5'- ------aGGUCACACCCAGGaauGUUUa -3' miRNA: 3'- auuuuugUCAGUGUGGGUCCgc-CAGA- -5' |
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9413 | 5' | -51.4 | NC_002531.1 | + | 12347 | 0.68 | 0.948784 |
Target: 5'- --uAGAgAG-CGCcUCCAGGCGGUCa -3' miRNA: 3'- auuUUUgUCaGUGuGGGUCCGCCAGa -5' |
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9413 | 5' | -51.4 | NC_002531.1 | + | 60730 | 0.68 | 0.944179 |
Target: 5'- --cAAGCAcaCACACCCAGGUGuGUUUu -3' miRNA: 3'- auuUUUGUcaGUGUGGGUCCGC-CAGA- -5' |
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9413 | 5' | -51.4 | NC_002531.1 | + | 13999 | 0.68 | 0.944179 |
Target: 5'- ----cAUAGUCACccuCCCAguGGUGGUCa -3' miRNA: 3'- auuuuUGUCAGUGu--GGGU--CCGCCAGa -5' |
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9413 | 5' | -51.4 | NC_002531.1 | + | 34019 | 0.68 | 0.934179 |
Target: 5'- aAAGGAC-GUgGCACCCaagcaaccuaGGGUGGUCa -3' miRNA: 3'- aUUUUUGuCAgUGUGGG----------UCCGCCAGa -5' |
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9413 | 5' | -51.4 | NC_002531.1 | + | 34675 | 0.68 | 0.92878 |
Target: 5'- -uGAGAUAccCGCugCCuGGCGGUCUa -3' miRNA: 3'- auUUUUGUcaGUGugGGuCCGCCAGA- -5' |
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9413 | 5' | -51.4 | NC_002531.1 | + | 477 | 0.69 | 0.917182 |
Target: 5'- cAAAGgcACAGaaACACCCGuGCGGUCUc -3' miRNA: 3'- aUUUU--UGUCagUGUGGGUcCGCCAGA- -5' |
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9413 | 5' | -51.4 | NC_002531.1 | + | 80792 | 0.7 | 0.883578 |
Target: 5'- aGAAggUAGUCGgcuCugCCAGGUGGUg- -3' miRNA: 3'- aUUUuuGUCAGU---GugGGUCCGCCAga -5' |
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9413 | 5' | -51.4 | NC_002531.1 | + | 20422 | 0.72 | 0.779351 |
Target: 5'- --cGAGCAGUCAUACuCCAGGUGcGUg- -3' miRNA: 3'- auuUUUGUCAGUGUG-GGUCCGC-CAga -5' |
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9413 | 5' | -51.4 | NC_002531.1 | + | 600 | 1.06 | 0.009295 |
Target: 5'- cUAAAAACAGUCACACCCAGGCGGUCUc -3' miRNA: 3'- -AUUUUUGUCAGUGUGGGUCCGCCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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