Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9414 | 3' | -49.6 | NC_002531.1 | + | 8198 | 0.68 | 0.970309 |
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Target: 5'- cUUCUGugcccucCGAGGGCUGuuugGGCAUGuGCa- -3' miRNA: 3'- aAAGAU-------GUUCCCGAU----UCGUACuCGac -5' |
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| 9414 | 3' | -49.6 | NC_002531.1 | + | 41361 | 0.69 | 0.951764 |
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Target: 5'- --cCUGCGAGGGagaAAGCAccUGAGCa- -3' miRNA: 3'- aaaGAUGUUCCCga-UUCGU--ACUCGac -5' |
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| 9414 | 3' | -49.6 | NC_002531.1 | + | 108210 | 0.69 | 0.951764 |
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Target: 5'- uUUUUUGCAGGGGUUucuacaaacUAUGAGCUGg -3' miRNA: 3'- -AAAGAUGUUCCCGAuuc------GUACUCGAC- -5' |
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| 9414 | 3' | -49.6 | NC_002531.1 | + | 67051 | 0.69 | 0.947195 |
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Target: 5'- --aCUGCAuaacGGGCacGGCggGAGCUGg -3' miRNA: 3'- aaaGAUGUu---CCCGauUCGuaCUCGAC- -5' |
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| 9414 | 3' | -49.6 | NC_002531.1 | + | 1083 | 0.69 | 0.94235 |
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Target: 5'- gUUUUUACAAGuGGuCUAGGCAccAGCUGu -3' miRNA: 3'- -AAAGAUGUUC-CC-GAUUCGUacUCGAC- -5' |
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| 9414 | 3' | -49.6 | NC_002531.1 | + | 12337 | 0.72 | 0.854029 |
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Target: 5'- -gUCUACAAcaGCUAcuaucugcAGCAUGGGCUGg -3' miRNA: 3'- aaAGAUGUUccCGAU--------UCGUACUCGAC- -5' |
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| 9414 | 3' | -49.6 | NC_002531.1 | + | 121519 | 0.74 | 0.726868 |
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Target: 5'- -cUCUGCAGGGGgccAGCAUGAuGCUGc -3' miRNA: 3'- aaAGAUGUUCCCgauUCGUACU-CGAC- -5' |
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| 9414 | 3' | -49.6 | NC_002531.1 | + | 1280 | 1.08 | 0.00845 |
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Target: 5'- aUUUCUACAAGGGCUAAGCAUGAGCUGg -3' miRNA: 3'- -AAAGAUGUUCCCGAUUCGUACUCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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