Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9416 | 3' | -53.6 | NC_002531.1 | + | 11395 | 0.66 | 0.961937 |
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Target: 5'- --gGCGggGGCUuuuucccucccgcUCCUCCACgGg--- -3' miRNA: 3'- ccaUGCuuCCGA-------------AGGAGGUGgCacug -5' |
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| 9416 | 3' | -53.6 | NC_002531.1 | + | 78376 | 0.66 | 0.954805 |
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Target: 5'- aGUAC-AAGGCcUCCUCCggguACCGUaGCu -3' miRNA: 3'- cCAUGcUUCCGaAGGAGG----UGGCAcUG- -5' |
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| 9416 | 3' | -53.6 | NC_002531.1 | + | 120013 | 0.67 | 0.931892 |
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Target: 5'- gGGgACGGAGGaaacuUUCCUCCAauucCCGcGGCa -3' miRNA: 3'- -CCaUGCUUCCg----AAGGAGGU----GGCaCUG- -5' |
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| 9416 | 3' | -53.6 | NC_002531.1 | + | 21144 | 0.67 | 0.926569 |
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Target: 5'- gGGUACcAGGGUg----CCACCGUGAUc -3' miRNA: 3'- -CCAUGcUUCCGaaggaGGUGGCACUG- -5' |
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| 9416 | 3' | -53.6 | NC_002531.1 | + | 122590 | 0.69 | 0.875129 |
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Target: 5'- --gACGGAGGCcUCguggCCugCGUGGCg -3' miRNA: 3'- ccaUGCUUCCGaAGga--GGugGCACUG- -5' |
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| 9416 | 3' | -53.6 | NC_002531.1 | + | 11585 | 1.11 | 0.003285 |
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Target: 5'- gGGUACGAAGGCUUCCUCCACCGUGACc -3' miRNA: 3'- -CCAUGCUUCCGAAGGAGGUGGCACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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