Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9417 | 5' | -44.3 | NC_002531.1 | + | 18565 | 0.66 | 0.999997 |
Target: 5'- cGUGAAgaccaugccAGUGgGUGacGUUGCCaCCCUg -3' miRNA: 3'- aCAUUU---------UCAUgUACaaCAAUGG-GGGG- -5' |
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9417 | 5' | -44.3 | NC_002531.1 | + | 7740 | 0.66 | 0.999997 |
Target: 5'- gGU-GAAGUAUGUGUgcgUGCCUgCCg -3' miRNA: 3'- aCAuUUUCAUGUACAacaAUGGGgGG- -5' |
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9417 | 5' | -44.3 | NC_002531.1 | + | 79231 | 0.66 | 0.999997 |
Target: 5'- gGUGAGGGUugGgcauugaagUGUUG-UGCCCaCUCu -3' miRNA: 3'- aCAUUUUCAugU---------ACAACaAUGGG-GGG- -5' |
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9417 | 5' | -44.3 | NC_002531.1 | + | 36906 | 0.66 | 0.999994 |
Target: 5'- gGUGAAGGUAaaaGUGaUGUUcugucugagccuaACCCCUUa -3' miRNA: 3'- aCAUUUUCAUg--UACaACAA-------------UGGGGGG- -5' |
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9417 | 5' | -44.3 | NC_002531.1 | + | 31749 | 0.67 | 0.999992 |
Target: 5'- --gGAGGGUugAUGUaucaGCCCUCCa -3' miRNA: 3'- acaUUUUCAugUACAacaaUGGGGGG- -5' |
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9417 | 5' | -44.3 | NC_002531.1 | + | 124361 | 0.67 | 0.999992 |
Target: 5'- aGU-AAAGUACAUGgc--UGCCCUCa -3' miRNA: 3'- aCAuUUUCAUGUACaacaAUGGGGGg -5' |
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9417 | 5' | -44.3 | NC_002531.1 | + | 95857 | 0.67 | 0.999989 |
Target: 5'- ----cGAGU--GUGUUGUgugUGCCCCCUu -3' miRNA: 3'- acauuUUCAugUACAACA---AUGGGGGG- -5' |
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9417 | 5' | -44.3 | NC_002531.1 | + | 13502 | 0.68 | 0.999958 |
Target: 5'- --gGGGAGUACAUGUUGUUgguggcgGCgUCCa -3' miRNA: 3'- acaUUUUCAUGUACAACAA-------UGgGGGg -5' |
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9417 | 5' | -44.3 | NC_002531.1 | + | 19313 | 0.68 | 0.999925 |
Target: 5'- uUGUAAGA--GCAUGUUGUacugACUUCCUg -3' miRNA: 3'- -ACAUUUUcaUGUACAACAa---UGGGGGG- -5' |
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9417 | 5' | -44.3 | NC_002531.1 | + | 14159 | 0.68 | 0.999925 |
Target: 5'- cGUaAAAAGUACcUGUUuaugACUCCCCu -3' miRNA: 3'- aCA-UUUUCAUGuACAAcaa-UGGGGGG- -5' |
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9417 | 5' | -44.3 | NC_002531.1 | + | 17399 | 0.68 | 0.999911 |
Target: 5'- cUGU-GAAGUcacagaaaccgaugcACGUGcUG-UGCCCCCCu -3' miRNA: 3'- -ACAuUUUCA---------------UGUACaACaAUGGGGGG- -5' |
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9417 | 5' | -44.3 | NC_002531.1 | + | 40261 | 0.68 | 0.999901 |
Target: 5'- -aUGAGGGggGCGga-UGUUACUCCCCg -3' miRNA: 3'- acAUUUUCa-UGUacaACAAUGGGGGG- -5' |
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9417 | 5' | -44.3 | NC_002531.1 | + | 52088 | 0.69 | 0.999714 |
Target: 5'- --cAAAGGga-GUGUUG-UGCCCCCUc -3' miRNA: 3'- acaUUUUCaugUACAACaAUGGGGGG- -5' |
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9417 | 5' | -44.3 | NC_002531.1 | + | 113315 | 0.69 | 0.999714 |
Target: 5'- gUGUAAAAaagACaAUGUUGUaaaGCCCCCa -3' miRNA: 3'- -ACAUUUUca-UG-UACAACAa--UGGGGGg -5' |
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9417 | 5' | -44.3 | NC_002531.1 | + | 11612 | 0.74 | 0.982437 |
Target: 5'- uUGUuuGGGUACGUGccuuucACCCCCCu -3' miRNA: 3'- -ACAuuUUCAUGUACaacaa-UGGGGGG- -5' |
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9417 | 5' | -44.3 | NC_002531.1 | + | 18682 | 1.15 | 0.01695 |
Target: 5'- uUGUAAAAGUACAUGUUGUUACCCCCCu -3' miRNA: 3'- -ACAUUUUCAUGUACAACAAUGGGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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