Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9418 | 5' | -58.8 | NC_002531.1 | + | 42708 | 0.66 | 0.760678 |
Target: 5'- aCAUgacGGCCAGugUaguaaagGCGC-UGGCUGGa -3' miRNA: 3'- gGUGa--CCGGUCugA-------CGCGuACCGGCU- -5' |
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9418 | 5' | -58.8 | NC_002531.1 | + | 94023 | 0.66 | 0.742514 |
Target: 5'- -aGCgGGCCAGGCUG-GUggGGCUGu -3' miRNA: 3'- ggUGaCCGGUCUGACgCGuaCCGGCu -5' |
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9418 | 5' | -58.8 | NC_002531.1 | + | 65766 | 0.67 | 0.71316 |
Target: 5'- gCGCUGGaCGGGCUGagccaGUcUGGCCGc -3' miRNA: 3'- gGUGACCgGUCUGACg----CGuACCGGCu -5' |
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9418 | 5' | -58.8 | NC_002531.1 | + | 82925 | 0.67 | 0.703227 |
Target: 5'- gCugUGGCCcc-CUGUgGCAUGuGCCGAu -3' miRNA: 3'- gGugACCGGucuGACG-CGUAC-CGGCU- -5' |
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9418 | 5' | -58.8 | NC_002531.1 | + | 35699 | 0.68 | 0.662991 |
Target: 5'- -gGCUGGCgGGguauguGCUGCGUA-GGCUGGg -3' miRNA: 3'- ggUGACCGgUC------UGACGCGUaCCGGCU- -5' |
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9418 | 5' | -58.8 | NC_002531.1 | + | 120257 | 0.68 | 0.662991 |
Target: 5'- gCC-CUGGCgaggaAGACUGCG-AUGGCgGGc -3' miRNA: 3'- -GGuGACCGg----UCUGACGCgUACCGgCU- -5' |
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9418 | 5' | -58.8 | NC_002531.1 | + | 57942 | 0.69 | 0.581839 |
Target: 5'- -aGCUGGCCGuccaGCgGCGCGUGGCgGu -3' miRNA: 3'- ggUGACCGGUc---UGaCGCGUACCGgCu -5' |
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9418 | 5' | -58.8 | NC_002531.1 | + | 24915 | 0.69 | 0.550804 |
Target: 5'- gCCACUGGUucaCAGG-UGCGCAgcuucacUGGCUGGu -3' miRNA: 3'- -GGUGACCG---GUCUgACGCGU-------ACCGGCU- -5' |
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9418 | 5' | -58.8 | NC_002531.1 | + | 21380 | 0.71 | 0.474453 |
Target: 5'- uCUGCUGGCCAGcCUGCugaaaaccuGguUGGCCa- -3' miRNA: 3'- -GGUGACCGGUCuGACG---------CguACCGGcu -5' |
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9418 | 5' | -58.8 | NC_002531.1 | + | 20232 | 0.71 | 0.474453 |
Target: 5'- uCCACUGGCCAGGCcuCGCGUGcaCCa- -3' miRNA: 3'- -GGUGACCGGUCUGacGCGUACc-GGcu -5' |
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9418 | 5' | -58.8 | NC_002531.1 | + | 20257 | 1.1 | 0.001055 |
Target: 5'- gCCACUGGCCAGACUGCGCAUGGCCGAg -3' miRNA: 3'- -GGUGACCGGUCUGACGCGUACCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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