Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9419 | 5' | -46.2 | NC_002531.1 | + | 17356 | 0.66 | 0.999609 |
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Target: 5'- -cGgGAUGGCUGCUGGgGGgauaucGCAGGa -3' miRNA: 3'- uaUgUUAUUGACGACUgUCa-----CGUUCa -5' |
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| 9419 | 5' | -46.2 | NC_002531.1 | + | 130094 | 0.67 | 0.999025 |
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Target: 5'- -aACcAUGACUGCaGACAGaUGCAGa- -3' miRNA: 3'- uaUGuUAUUGACGaCUGUC-ACGUUca -5' |
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| 9419 | 5' | -46.2 | NC_002531.1 | + | 12682 | 0.67 | 0.999025 |
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Target: 5'- gGUACucuucuGgUGCUGGCAGaacUGCAGGUa -3' miRNA: 3'- -UAUGuuau--UgACGACUGUC---ACGUUCA- -5' |
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| 9419 | 5' | -46.2 | NC_002531.1 | + | 88653 | 0.68 | 0.997365 |
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Target: 5'- uGUACAGgagGGCUGCUGcccuGguGUGUAGGa -3' miRNA: 3'- -UAUGUUa--UUGACGAC----UguCACGUUCa -5' |
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| 9419 | 5' | -46.2 | NC_002531.1 | + | 44792 | 0.7 | 0.990311 |
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Target: 5'- uGUACAGUGACgGCcuuugaaacUGGCAGUGCGc-- -3' miRNA: 3'- -UAUGUUAUUGaCG---------ACUGUCACGUuca -5' |
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| 9419 | 5' | -46.2 | NC_002531.1 | + | 89277 | 0.72 | 0.966722 |
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Target: 5'- -cACAGaaguUAGCUaCUGGCAGUGCAGGc -3' miRNA: 3'- uaUGUU----AUUGAcGACUGUCACGUUCa -5' |
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| 9419 | 5' | -46.2 | NC_002531.1 | + | 29162 | 1.07 | 0.022394 |
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Target: 5'- aAUACAAUAACUGCUGACAGUGCAAGUa -3' miRNA: 3'- -UAUGUUAUUGACGACUGUCACGUUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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