Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9421 | 5' | -53.4 | NC_002531.1 | + | 49632 | 0.66 | 0.930129 |
Target: 5'- --cUGUAggucAGUGCAgcuGGUGGCCgcauagccagcgaGCCUCu -3' miRNA: 3'- guuACGU----UCACGU---UCACCGGa------------CGGAG- -5' |
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9421 | 5' | -53.4 | NC_002531.1 | + | 35808 | 0.66 | 0.91506 |
Target: 5'- --cUGCAGGuUGCugauGGUGGCggcuuuCUGCCUg -3' miRNA: 3'- guuACGUUC-ACGu---UCACCG------GACGGAg -5' |
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9421 | 5' | -53.4 | NC_002531.1 | + | 74820 | 0.67 | 0.908789 |
Target: 5'- ---gGgAGGUGCAGGU-GCCUGCUg- -3' miRNA: 3'- guuaCgUUCACGUUCAcCGGACGGag -5' |
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9421 | 5' | -53.4 | NC_002531.1 | + | 91529 | 0.67 | 0.902256 |
Target: 5'- ------uGGUGCAGaaGGCCUGCCUUg -3' miRNA: 3'- guuacguUCACGUUcaCCGGACGGAG- -5' |
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9421 | 5' | -53.4 | NC_002531.1 | + | 129720 | 0.68 | 0.841067 |
Target: 5'- aGGUGCAAGUGgGAGaaguccaaaUGGCCUaggagcucgGCCUa -3' miRNA: 3'- gUUACGUUCACgUUC---------ACCGGA---------CGGAg -5' |
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9421 | 5' | -53.4 | NC_002531.1 | + | 29875 | 0.68 | 0.832394 |
Target: 5'- aAAUGCAGGUGaGAGauaGCCUGCUUUa -3' miRNA: 3'- gUUACGUUCACgUUCac-CGGACGGAG- -5' |
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9421 | 5' | -53.4 | NC_002531.1 | + | 63981 | 0.7 | 0.773484 |
Target: 5'- aAAUGCAGGUGUuuGUGGUuuuaaaaggaacugCUGCCa- -3' miRNA: 3'- gUUACGUUCACGuuCACCG--------------GACGGag -5' |
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9421 | 5' | -53.4 | NC_002531.1 | + | 27639 | 0.7 | 0.73613 |
Target: 5'- ---aGUAAGUGCGAGUaaguacugauGGCUUGUCUUg -3' miRNA: 3'- guuaCGUUCACGUUCA----------CCGGACGGAG- -5' |
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9421 | 5' | -53.4 | NC_002531.1 | + | 124834 | 0.71 | 0.683571 |
Target: 5'- gUAAUGCGGGUGgAAGuUGGCaggUGCCUg -3' miRNA: 3'- -GUUACGUUCACgUUC-ACCGg--ACGGAg -5' |
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9421 | 5' | -53.4 | NC_002531.1 | + | 36563 | 0.72 | 0.640608 |
Target: 5'- aCAGUGCcuucAAGUGC-AGUcGCCUGCCgUCg -3' miRNA: 3'- -GUUACG----UUCACGuUCAcCGGACGG-AG- -5' |
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9421 | 5' | -53.4 | NC_002531.1 | + | 29998 | 1.1 | 0.002584 |
Target: 5'- gCAAUGCAAGUGCAAGUGGCCUGCCUCa -3' miRNA: 3'- -GUUACGUUCACGUUCACCGGACGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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