Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9423 | 3' | -58 | NC_002531.1 | + | 53149 | 0.66 | 0.769785 |
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Target: 5'- aUGGGGgAGGGguuGGUGUgcgagcuaacaggccGCUguGCAGu -3' miRNA: 3'- gACCCCgUCCC---UCACAa--------------CGAguCGUC- -5' |
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| 9423 | 3' | -58 | NC_002531.1 | + | 53355 | 0.66 | 0.765974 |
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Target: 5'- aUGGGGCGGGGGuuuccaaaacuuacGcUGUUGaCUgcaCAGCGGc -3' miRNA: 3'- gACCCCGUCCCU--------------C-ACAAC-GA---GUCGUC- -5' |
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| 9423 | 3' | -58 | NC_002531.1 | + | 34546 | 0.66 | 0.749564 |
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Target: 5'- -gGGGGCGGGGGGU---GCUCcagGGCc- -3' miRNA: 3'- gaCCCCGUCCCUCAcaaCGAG---UCGuc -5' |
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| 9423 | 3' | -58 | NC_002531.1 | + | 95604 | 0.67 | 0.719894 |
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Target: 5'- -gGaGGGCucuGGGGUGUauUGCUCAGUAa -3' miRNA: 3'- gaC-CCCGuc-CCUCACA--ACGAGUCGUc -5' |
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| 9423 | 3' | -58 | NC_002531.1 | + | 294 | 0.68 | 0.617395 |
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Target: 5'- aCUGGGGCuGGGAGaGggGCUaucugcugcgCGGCGc -3' miRNA: 3'- -GACCCCGuCCCUCaCaaCGA----------GUCGUc -5' |
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| 9423 | 3' | -58 | NC_002531.1 | + | 32494 | 1.07 | 0.001672 |
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Target: 5'- gCUGGGGCAGGGAGUGUUGCUCAGCAGc -3' miRNA: 3'- -GACCCCGUCCCUCACAACGAGUCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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