Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9423 | 5' | -60 | NC_002531.1 | + | 33840 | 0.66 | 0.681263 |
Target: 5'- gGCUGaaAGCuGgCAUUgACCACCCUAg -3' miRNA: 3'- aCGACgcUCG-CgGUGAgUGGUGGGAU- -5' |
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9423 | 5' | -60 | NC_002531.1 | + | 29060 | 0.66 | 0.671087 |
Target: 5'- gUGCucaacaUGCGuGCGCCAaaCACCcCCCUc -3' miRNA: 3'- -ACG------ACGCuCGCGGUgaGUGGuGGGAu -5' |
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9423 | 5' | -60 | NC_002531.1 | + | 68032 | 0.66 | 0.650647 |
Target: 5'- aGCaGUGAauGUGCCuucuCUCACCcCCCUGg -3' miRNA: 3'- aCGaCGCU--CGCGGu---GAGUGGuGGGAU- -5' |
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9423 | 5' | -60 | NC_002531.1 | + | 113466 | 0.66 | 0.64655 |
Target: 5'- gUGCUGUuuGAGUggcagccgcaccaGCCACUCGCCGCagacggucagccauCCUGa -3' miRNA: 3'- -ACGACG--CUCG-------------CGGUGAGUGGUG--------------GGAU- -5' |
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9423 | 5' | -60 | NC_002531.1 | + | 81846 | 0.66 | 0.630148 |
Target: 5'- cUGCUGCGGGCuGUgGCUUuuugggaaGCCuACCCa- -3' miRNA: 3'- -ACGACGCUCG-CGgUGAG--------UGG-UGGGau -5' |
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9423 | 5' | -60 | NC_002531.1 | + | 69525 | 0.67 | 0.568928 |
Target: 5'- -----gGGGCGUCGCUUGCCACCCa- -3' miRNA: 3'- acgacgCUCGCGGUGAGUGGUGGGau -5' |
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9423 | 5' | -60 | NC_002531.1 | + | 113233 | 0.67 | 0.568928 |
Target: 5'- gUGCUGCG-GCGCgAaaaGCCACCUg- -3' miRNA: 3'- -ACGACGCuCGCGgUgagUGGUGGGau -5' |
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9423 | 5' | -60 | NC_002531.1 | + | 113297 | 0.68 | 0.547805 |
Target: 5'- cUGCgGCGAGUggcuggugcggcuGCCACUCAaacagCACCCa- -3' miRNA: 3'- -ACGaCGCUCG-------------CGGUGAGUg----GUGGGau -5' |
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9423 | 5' | -60 | NC_002531.1 | + | 82074 | 0.68 | 0.52891 |
Target: 5'- ---aGCGcAGCGCCcCUCugCACCCc- -3' miRNA: 3'- acgaCGC-UCGCGGuGAGugGUGGGau -5' |
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9423 | 5' | -60 | NC_002531.1 | + | 103723 | 0.68 | 0.519062 |
Target: 5'- gUGCagGCGccuccGGCGCCGC-CACCACUCc- -3' miRNA: 3'- -ACGa-CGC-----UCGCGGUGaGUGGUGGGau -5' |
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9423 | 5' | -60 | NC_002531.1 | + | 100878 | 0.69 | 0.489976 |
Target: 5'- gUGCUGCaGGC-CCAUuuuggaaccaUCGCCACCCa- -3' miRNA: 3'- -ACGACGcUCGcGGUG----------AGUGGUGGGau -5' |
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9423 | 5' | -60 | NC_002531.1 | + | 86726 | 0.75 | 0.198133 |
Target: 5'- aGCUGgGGuuucucGCGUCGCUCACCAuCCCUAc -3' miRNA: 3'- aCGACgCU------CGCGGUGAGUGGU-GGGAU- -5' |
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9423 | 5' | -60 | NC_002531.1 | + | 32459 | 1.06 | 0.001237 |
Target: 5'- cUGCUGCGAGCGCCACUCACCACCCUAg -3' miRNA: 3'- -ACGACGCUCGCGGUGAGUGGUGGGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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