Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9424 | 3' | -49.5 | NC_002531.1 | + | 11385 | 0.66 | 0.997102 |
Target: 5'- -aGUAUgccGAGGCGGGgGCUuuuUCCCu -3' miRNA: 3'- ugUAUAaauUUCCGCUCgCGGc--AGGG- -5' |
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9424 | 3' | -49.5 | NC_002531.1 | + | 58771 | 0.66 | 0.997102 |
Target: 5'- ---aGUUUAAAGGCcuaacAGaCGCCGUCUUu -3' miRNA: 3'- uguaUAAAUUUCCGc----UC-GCGGCAGGG- -5' |
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9424 | 3' | -49.5 | NC_002531.1 | + | 84984 | 0.66 | 0.994458 |
Target: 5'- --cUGUgguuuGGCG-GCGCUGUCCUu -3' miRNA: 3'- uguAUAaauuuCCGCuCGCGGCAGGG- -5' |
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9424 | 3' | -49.5 | NC_002531.1 | + | 90326 | 0.67 | 0.993558 |
Target: 5'- gUAUGUUUuuuccAGGGGCGAGUGaCUGUCg- -3' miRNA: 3'- uGUAUAAA-----UUUCCGCUCGC-GGCAGgg -5' |
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9424 | 3' | -49.5 | NC_002531.1 | + | 24971 | 0.67 | 0.993558 |
Target: 5'- aGCAUAcgucucggGAAGGCagguGAGCGUCGUUgCa -3' miRNA: 3'- -UGUAUaaa-----UUUCCG----CUCGCGGCAGgG- -5' |
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9424 | 3' | -49.5 | NC_002531.1 | + | 13496 | 0.68 | 0.98716 |
Target: 5'- uACAUGUUguu-GGUG-GCGgCGUCCa -3' miRNA: 3'- -UGUAUAAauuuCCGCuCGCgGCAGGg -5' |
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9424 | 3' | -49.5 | NC_002531.1 | + | 108717 | 0.68 | 0.985433 |
Target: 5'- aGCAguac--AAGaGCGAGgGuuGUCCCa -3' miRNA: 3'- -UGUauaaauUUC-CGCUCgCggCAGGG- -5' |
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9424 | 3' | -49.5 | NC_002531.1 | + | 51742 | 0.68 | 0.983534 |
Target: 5'- cGCAUGgu---AGGUGuGGCuGCCGUCUCu -3' miRNA: 3'- -UGUAUaaauuUCCGC-UCG-CGGCAGGG- -5' |
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9424 | 3' | -49.5 | NC_002531.1 | + | 120122 | 0.71 | 0.939312 |
Target: 5'- --------cAGGGCGAGUGCCcaGUCCUg -3' miRNA: 3'- uguauaaauUUCCGCUCGCGG--CAGGG- -5' |
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9424 | 3' | -49.5 | NC_002531.1 | + | 115862 | 0.72 | 0.890814 |
Target: 5'- uAUGUAggUGGGGGCuGGCcCCGUCCCc -3' miRNA: 3'- -UGUAUaaAUUUCCGcUCGcGGCAGGG- -5' |
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9424 | 3' | -49.5 | NC_002531.1 | + | 34611 | 0.75 | 0.759463 |
Target: 5'- ---gAUUUAAGGGCGc-CGCCGUCUCa -3' miRNA: 3'- uguaUAAAUUUCCGCucGCGGCAGGG- -5' |
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9424 | 3' | -49.5 | NC_002531.1 | + | 34799 | 1.14 | 0.005327 |
Target: 5'- cACAUAUUUAAAGGCGAGCGCCGUCCCa -3' miRNA: 3'- -UGUAUAAAUUUCCGCUCGCGGCAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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