miRNA: 3'- aaCuCUGCC-GCCGCGGGaauuua-GUC- -5'
miRNA: 3'- aACUCUGcc--------GCCGCGGGAAUUUaGUC- -5'
miRNA: 3'- aACUCUGCCGCc-GCGGGAAUUUAguc -5'
miRNA: 3'- aaCUCUGCCgCCGCGGgaAUUUa--GUC- -5'
miRNA: 3'- -AACUCUGCCGCCGCGGGAAUUUAGUC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9424 | 5' | -53.8 | NC_002531.1 | + | 45918 | 0.65 | 0.940874 |
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Target: 5'- -aGuGAUGGaCGGCGCCCcaacaagCAGa -3' miRNA: 3'- aaCuCUGCC-GCCGCGGGaauuua-GUC- -5' |
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| 9424 | 5' | -53.8 | NC_002531.1 | + | 93105 | 0.66 | 0.936922 |
|
Target: 5'- cUGAGACaugcauucaaCGGCGCUCUUGAG-CAGg -3' miRNA: 3'- aACUCUGcc--------GCCGCGGGAAUUUaGUC- -5' |
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| 9424 | 5' | -53.8 | NC_002531.1 | + | 34641 | 0.72 | 0.65483 |
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Target: 5'- gUGGGACGGCGcuCGCCUUUAAAUa-- -3' miRNA: 3'- aACUCUGCCGCc-GCGGGAAUUUAguc -5' |
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| 9424 | 5' | -53.8 | NC_002531.1 | + | 12565 | 0.73 | 0.601665 |
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Target: 5'- -gGAGGCGGgGGCGCCggUAAAggcCGGg -3' miRNA: 3'- aaCUCUGCCgCCGCGGgaAUUUa--GUC- -5' |
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| 9424 | 5' | -53.8 | NC_002531.1 | + | 34765 | 1.05 | 0.005643 |
|
Target: 5'- cUUGAGACGGCGGCGCCCUUAAAUCAGc -3' miRNA: 3'- -AACUCUGCCGCCGCGGGAAUUUAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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