Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9425 | 3' | -50.4 | NC_002531.1 | + | 11589 | 0.66 | 0.992225 |
Target: 5'- cGUUGGGUACGAAGGcuuccuccaccGUGAcCCCGuGg -3' miRNA: 3'- aCGAUCCGUGUUUUC-----------UACUuGGGC-Cg -5' |
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9425 | 3' | -50.4 | NC_002531.1 | + | 103431 | 0.66 | 0.992225 |
Target: 5'- -cCUGGGCugGAGgccuggccuccAGAguuaucaGACCCGGCu -3' miRNA: 3'- acGAUCCGugUUU-----------UCUac-----UUGGGCCG- -5' |
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9425 | 3' | -50.4 | NC_002531.1 | + | 54255 | 0.66 | 0.992225 |
Target: 5'- gGC-AGGCGCGccGAAccgcccuaGUGAACCUGGUg -3' miRNA: 3'- aCGaUCCGUGU--UUUc-------UACUUGGGCCG- -5' |
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9425 | 3' | -50.4 | NC_002531.1 | + | 12466 | 0.66 | 0.991048 |
Target: 5'- -aUUGGGCuCAAcuuuggaauuaGGGAUGGggGCCUGGCg -3' miRNA: 3'- acGAUCCGuGUU-----------UUCUACU--UGGGCCG- -5' |
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9425 | 3' | -50.4 | NC_002531.1 | + | 6310 | 0.66 | 0.989735 |
Target: 5'- uUGCUAaaUGCAGcAAGAUGAucACCCGGg -3' miRNA: 3'- -ACGAUccGUGUU-UUCUACU--UGGGCCg -5' |
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9425 | 3' | -50.4 | NC_002531.1 | + | 44077 | 0.66 | 0.989735 |
Target: 5'- aGCcauggAGGCGgGGAAGAUGucagucaCUGGCa -3' miRNA: 3'- aCGa----UCCGUgUUUUCUACuug----GGCCG- -5' |
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9425 | 3' | -50.4 | NC_002531.1 | + | 40271 | 0.66 | 0.988277 |
Target: 5'- gGCU-GGUACGAuGAGggGGGcggauguuacucCCCGGCg -3' miRNA: 3'- aCGAuCCGUGUU-UUCuaCUU------------GGGCCG- -5' |
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9425 | 3' | -50.4 | NC_002531.1 | + | 67187 | 0.66 | 0.986662 |
Target: 5'- aUGCagUAGGUugAGAAGGgcaggcggGGGCCCaGGUc -3' miRNA: 3'- -ACG--AUCCGugUUUUCUa-------CUUGGG-CCG- -5' |
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9425 | 3' | -50.4 | NC_002531.1 | + | 121757 | 0.66 | 0.986662 |
Target: 5'- aGgaGGGCGC---GGA-GAGCCgGGCu -3' miRNA: 3'- aCgaUCCGUGuuuUCUaCUUGGgCCG- -5' |
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9425 | 3' | -50.4 | NC_002531.1 | + | 15351 | 0.67 | 0.984882 |
Target: 5'- gUGCgAGGCACAGGGGAUauACCUcucaGCu -3' miRNA: 3'- -ACGaUCCGUGUUUUCUAcuUGGGc---CG- -5' |
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9425 | 3' | -50.4 | NC_002531.1 | + | 108564 | 0.67 | 0.978452 |
Target: 5'- uUGCUGGGC-Cu--GGGUGAAUCCa-- -3' miRNA: 3'- -ACGAUCCGuGuuuUCUACUUGGGccg -5' |
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9425 | 3' | -50.4 | NC_002531.1 | + | 82878 | 0.68 | 0.966995 |
Target: 5'- aGUUuaGGGguCu---GAUGGACCUGGCg -3' miRNA: 3'- aCGA--UCCguGuuuuCUACUUGGGCCG- -5' |
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9425 | 3' | -50.4 | NC_002531.1 | + | 9569 | 0.68 | 0.959885 |
Target: 5'- ---cAGGCGC-GAAGAUGcugcACCUGGCu -3' miRNA: 3'- acgaUCCGUGuUUUCUACu---UGGGCCG- -5' |
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9425 | 3' | -50.4 | NC_002531.1 | + | 38478 | 0.69 | 0.94735 |
Target: 5'- gGCagAGGUuCAAGGGGgucAACCCGGCc -3' miRNA: 3'- aCGa-UCCGuGUUUUCUac-UUGGGCCG- -5' |
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9425 | 3' | -50.4 | NC_002531.1 | + | 59847 | 0.69 | 0.942655 |
Target: 5'- gUGC-AGGCguGCAcuGAGAUGAgaccccacAUCCGGCa -3' miRNA: 3'- -ACGaUCCG--UGUu-UUCUACU--------UGGGCCG- -5' |
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9425 | 3' | -50.4 | NC_002531.1 | + | 34839 | 0.7 | 0.908893 |
Target: 5'- gGCUcaGGGacuggaaGCGAGAGA-GGACCCaGGCg -3' miRNA: 3'- aCGA--UCCg------UGUUUUCUaCUUGGG-CCG- -5' |
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9425 | 3' | -50.4 | NC_002531.1 | + | 88881 | 0.71 | 0.902334 |
Target: 5'- cUGUUgAGGguCAAGGGAgUGAuGCCCGGUg -3' miRNA: 3'- -ACGA-UCCguGUUUUCU-ACU-UGGGCCG- -5' |
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9425 | 3' | -50.4 | NC_002531.1 | + | 12604 | 0.71 | 0.872757 |
Target: 5'- gGCUAGGUGCuGGAGGGUGAaagagcuGCCaucgaGGCu -3' miRNA: 3'- aCGAUCCGUG-UUUUCUACU-------UGGg----CCG- -5' |
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9425 | 3' | -50.4 | NC_002531.1 | + | 98216 | 0.73 | 0.814108 |
Target: 5'- -uCUGGGCcCAGGAGAgu--CCCGGCa -3' miRNA: 3'- acGAUCCGuGUUUUCUacuuGGGCCG- -5' |
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9425 | 3' | -50.4 | NC_002531.1 | + | 24383 | 0.75 | 0.708137 |
Target: 5'- aUGCUGGGC-CAGAAGAcUGcccgccuaaccuuuaGACCCGGg -3' miRNA: 3'- -ACGAUCCGuGUUUUCU-AC---------------UUGGGCCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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