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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9426 | 5' | -59.1 | NC_002531.1 | + | 129752 | 0.66 | 0.750421 |
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Target: 5'- gGUCCAGAuucaaugucAACCCCCGgugGUgGAGGUg -3' miRNA: 3'- aCGGGUCU---------UUGGGGGCg--CGgCUUCAg -5' |
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| 9426 | 5' | -59.1 | NC_002531.1 | + | 130021 | 0.67 | 0.681353 |
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Target: 5'- gUGCCCccGGAccuCCCCCG-GCCGccccGUCa -3' miRNA: 3'- -ACGGG--UCUuu-GGGGGCgCGGCuu--CAG- -5' |
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| 9426 | 5' | -59.1 | NC_002531.1 | + | 57204 | 0.67 | 0.661126 |
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Target: 5'- aUGCUCaAGGucaucagcAACCCCCGCcuGCCGGcgugaGGUCc -3' miRNA: 3'- -ACGGG-UCU--------UUGGGGGCG--CGGCU-----UCAG- -5' |
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| 9426 | 5' | -59.1 | NC_002531.1 | + | 125816 | 0.7 | 0.50101 |
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Target: 5'- aGCaacucugUAGAAGCCCCCGaGCCuGAGUCa -3' miRNA: 3'- aCGg------GUCUUUGGGGGCgCGGcUUCAG- -5' |
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| 9426 | 5' | -59.1 | NC_002531.1 | + | 35178 | 1.08 | 0.001378 |
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Target: 5'- cUGCCCAGAAACCCCCGCGCCGAAGUCc -3' miRNA: 3'- -ACGGGUCUUUGGGGGCGCGGCUUCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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