Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9428 | 3' | -61.1 | NC_002531.1 | + | 35696 | 0.67 | 0.567908 |
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Target: 5'- aAGGGCUggcgggguaugugCUGCguaggcUGGGCCUGUgCCAa -3' miRNA: 3'- gUCCCGA-------------GAUGaa----GCCCGGACGgGGU- -5' |
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| 9428 | 3' | -61.1 | NC_002531.1 | + | 29948 | 0.67 | 0.539237 |
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Target: 5'- aAGGGCUCcaagUugUUuugagUGGGCCUGCUCa- -3' miRNA: 3'- gUCCCGAG----AugAA-----GCCCGGACGGGgu -5' |
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| 9428 | 3' | -61.1 | NC_002531.1 | + | 105401 | 0.69 | 0.46314 |
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Target: 5'- aAGGGCUCUuggaaaGGuguuCCUGCCCCAg -3' miRNA: 3'- gUCCCGAGAugaag-CCc---GGACGGGGU- -5' |
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| 9428 | 3' | -61.1 | NC_002531.1 | + | 103847 | 0.69 | 0.427246 |
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Target: 5'- cCAGGuGCauUUUGa---GGGCCUGCCCCGg -3' miRNA: 3'- -GUCC-CG--AGAUgaagCCCGGACGGGGU- -5' |
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| 9428 | 3' | -61.1 | NC_002531.1 | + | 56632 | 0.7 | 0.409929 |
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Target: 5'- -cGGGCUguguagCUACUcuucggUGGGUUUGCCCCAg -3' miRNA: 3'- guCCCGA------GAUGAa-----GCCCGGACGGGGU- -5' |
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| 9428 | 3' | -61.1 | NC_002531.1 | + | 82522 | 0.7 | 0.384788 |
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Target: 5'- cCAGGGCUggagACUgCGuGGCCacgGCCCCGa -3' miRNA: 3'- -GUCCCGAga--UGAaGC-CCGGa--CGGGGU- -5' |
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| 9428 | 3' | -61.1 | NC_002531.1 | + | 58482 | 0.7 | 0.368604 |
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Target: 5'- gGGGGCau--CUUgGGGCUUGCUCCAg -3' miRNA: 3'- gUCCCGagauGAAgCCCGGACGGGGU- -5' |
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| 9428 | 3' | -61.1 | NC_002531.1 | + | 36003 | 1.08 | 0.000876 |
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Target: 5'- cCAGGGCUCUACUUCGGGCCUGCCCCAa -3' miRNA: 3'- -GUCCCGAGAUGAAGCCCGGACGGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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