Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9428 | 5' | -53.7 | NC_002531.1 | + | 113269 | 0.66 | 0.943349 |
Target: 5'- uGGCAGGAauaucaggauGGCUG--ACCGUCuGCGGCg -3' miRNA: 3'- -CCGUCUU----------UCGGCggUGGUAGuCGUUG- -5' |
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9428 | 5' | -53.7 | NC_002531.1 | + | 83555 | 0.66 | 0.943349 |
Target: 5'- cGUAGAAGGCCGCCAgC-UC-GCcuCg -3' miRNA: 3'- cCGUCUUUCGGCGGUgGuAGuCGuuG- -5' |
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9428 | 5' | -53.7 | NC_002531.1 | + | 11548 | 0.66 | 0.942878 |
Target: 5'- aGCGGGAgggaaaaAGCCcCCGCC-UCGGCAu- -3' miRNA: 3'- cCGUCUU-------UCGGcGGUGGuAGUCGUug -5' |
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9428 | 5' | -53.7 | NC_002531.1 | + | 83047 | 0.66 | 0.933442 |
Target: 5'- cGGUccguGAuGGCCGCCAgguCCAUCAGaccccuaAACu -3' miRNA: 3'- -CCGu---CUuUCGGCGGU---GGUAGUCg------UUG- -5' |
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9428 | 5' | -53.7 | NC_002531.1 | + | 54405 | 0.66 | 0.933442 |
Target: 5'- gGGCGGuucGGCgCGCCugCcgGUUAGCGGg -3' miRNA: 3'- -CCGUCuu-UCG-GCGGugG--UAGUCGUUg -5' |
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9428 | 5' | -53.7 | NC_002531.1 | + | 56363 | 0.66 | 0.933442 |
Target: 5'- uGGaUAGuu-GUgGCCAUCAUCAGCAu- -3' miRNA: 3'- -CC-GUCuuuCGgCGGUGGUAGUCGUug -5' |
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9428 | 5' | -53.7 | NC_002531.1 | + | 68764 | 0.66 | 0.922507 |
Target: 5'- uGGCAucuGAuaucucguGGCUGCCuCCAUuCGGCAAUg -3' miRNA: 3'- -CCGU---CUu-------UCGGCGGuGGUA-GUCGUUG- -5' |
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9428 | 5' | -53.7 | NC_002531.1 | + | 42707 | 0.67 | 0.916653 |
Target: 5'- uGCAGAgcGCCuGCCACCccUGGCuGACg -3' miRNA: 3'- cCGUCUuuCGG-CGGUGGuaGUCG-UUG- -5' |
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9428 | 5' | -53.7 | NC_002531.1 | + | 7244 | 0.68 | 0.87623 |
Target: 5'- uGUGGAugGGGCCaGCaagaACUGUCAGCAACu -3' miRNA: 3'- cCGUCU--UUCGG-CGg---UGGUAGUCGUUG- -5' |
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9428 | 5' | -53.7 | NC_002531.1 | + | 122969 | 0.68 | 0.860827 |
Target: 5'- aGGCcccguugcugGGAGAcGCgGCCACCuUCAGgGGCu -3' miRNA: 3'- -CCG----------UCUUU-CGgCGGUGGuAGUCgUUG- -5' |
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9428 | 5' | -53.7 | NC_002531.1 | + | 122423 | 0.68 | 0.844535 |
Target: 5'- aGGCAGAGcuCCGCCACgCA--GGCcACg -3' miRNA: 3'- -CCGUCUUucGGCGGUG-GUagUCGuUG- -5' |
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9428 | 5' | -53.7 | NC_002531.1 | + | 19954 | 0.69 | 0.827411 |
Target: 5'- aGGgGGuauGCUGUCACaccuGUCAGCAGCa -3' miRNA: 3'- -CCgUCuuuCGGCGGUGg---UAGUCGUUG- -5' |
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9428 | 5' | -53.7 | NC_002531.1 | + | 41272 | 0.69 | 0.818558 |
Target: 5'- aGCAGAAAGCCuGUUaauGCCAUCAGaaucaaAGCc -3' miRNA: 3'- cCGUCUUUCGG-CGG---UGGUAGUCg-----UUG- -5' |
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9428 | 5' | -53.7 | NC_002531.1 | + | 54668 | 0.69 | 0.800312 |
Target: 5'- uGGguGAAAGCCGCUgugGCUAcUCAGUcccggaAACa -3' miRNA: 3'- -CCguCUUUCGGCGG---UGGU-AGUCG------UUG- -5' |
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9428 | 5' | -53.7 | NC_002531.1 | + | 74447 | 0.69 | 0.800312 |
Target: 5'- -cCAGuAAGCCuGCgACCAgccgCAGCAGCa -3' miRNA: 3'- ccGUCuUUCGG-CGgUGGUa---GUCGUUG- -5' |
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9428 | 5' | -53.7 | NC_002531.1 | + | 69326 | 0.71 | 0.73181 |
Target: 5'- uGCAGAgcGCCGCCACgAUguGUuuGCu -3' miRNA: 3'- cCGUCUuuCGGCGGUGgUAguCGu-UG- -5' |
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9428 | 5' | -53.7 | NC_002531.1 | + | 47411 | 0.71 | 0.690391 |
Target: 5'- aGCccuGAAGGCCGCC-UCuUCGGCAGCc -3' miRNA: 3'- cCGu--CUUUCGGCGGuGGuAGUCGUUG- -5' |
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9428 | 5' | -53.7 | NC_002531.1 | + | 97963 | 0.72 | 0.658741 |
Target: 5'- aGGUAGAGAGCauguuacagGCCGCCAagUCAGaacaGACa -3' miRNA: 3'- -CCGUCUUUCGg--------CGGUGGU--AGUCg---UUG- -5' |
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9428 | 5' | -53.7 | NC_002531.1 | + | 8983 | 0.73 | 0.604592 |
Target: 5'- aGGCAGAAaaccuAGCUcucucugGCCACgauGUCAGCGACg -3' miRNA: 3'- -CCGUCUU-----UCGG-------CGGUGg--UAGUCGUUG- -5' |
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9428 | 5' | -53.7 | NC_002531.1 | + | 35969 | 1.11 | 0.00242 |
Target: 5'- aGGCAGAAAGCCGCCACCAUCAGCAACc -3' miRNA: 3'- -CCGUCUUUCGGCGGUGGUAGUCGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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