Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9430 | 5' | -50 | NC_002531.1 | + | 30228 | 0.66 | 0.994324 |
Target: 5'- uACGuaGCUCCACgGAGU-CCUG-GCuGCg -3' miRNA: 3'- -UGC--UGAGGUGgUUUAuGGACuCGuUG- -5' |
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9430 | 5' | -50 | NC_002531.1 | + | 11864 | 0.66 | 0.993401 |
Target: 5'- -aGGCUCUACC---UGCCUGcGGuCAGCg -3' miRNA: 3'- ugCUGAGGUGGuuuAUGGAC-UC-GUUG- -5' |
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9430 | 5' | -50 | NC_002531.1 | + | 18149 | 0.66 | 0.992792 |
Target: 5'- aACGugUUggaagagcuaucccgCGCguGGUGCCgUGAGCAGCa -3' miRNA: 3'- -UGCugAG---------------GUGguUUAUGG-ACUCGUUG- -5' |
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9430 | 5' | -50 | NC_002531.1 | + | 38262 | 0.66 | 0.992471 |
Target: 5'- -aGGCUauugagggcagcacaCGCCGAuUGCCcGAGCAGCg -3' miRNA: 3'- ugCUGAg--------------GUGGUUuAUGGaCUCGUUG- -5' |
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9430 | 5' | -50 | NC_002531.1 | + | 9826 | 0.66 | 0.989893 |
Target: 5'- -aGACUCCuuuucuucuACCAAAUGCCagcagGAGguAUg -3' miRNA: 3'- ugCUGAGG---------UGGUUUAUGGa----CUCguUG- -5' |
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9430 | 5' | -50 | NC_002531.1 | + | 130097 | 0.66 | 0.989893 |
Target: 5'- cAUGACUgCAgaCAGAUGCa-GAGCAACu -3' miRNA: 3'- -UGCUGAgGUg-GUUUAUGgaCUCGUUG- -5' |
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9430 | 5' | -50 | NC_002531.1 | + | 67881 | 0.67 | 0.98798 |
Target: 5'- cGCGGCUCUuucuaucCCAcucagaggugaagcAGUACCUGGGCucacuGCa -3' miRNA: 3'- -UGCUGAGGu------GGU--------------UUAUGGACUCGu----UG- -5' |
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9430 | 5' | -50 | NC_002531.1 | + | 55327 | 0.67 | 0.986841 |
Target: 5'- -gGACccagagCCACCAAauguuAUACCUGAG-AACa -3' miRNA: 3'- ugCUGa-----GGUGGUU-----UAUGGACUCgUUG- -5' |
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9430 | 5' | -50 | NC_002531.1 | + | 71661 | 0.67 | 0.986841 |
Target: 5'- aAC-ACUUCACCuugucuuaagcAAGUGUCUGAGCAGCa -3' miRNA: 3'- -UGcUGAGGUGG-----------UUUAUGGACUCGUUG- -5' |
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9430 | 5' | -50 | NC_002531.1 | + | 129986 | 0.67 | 0.983124 |
Target: 5'- uACGugcuacCUCCACCuu-UACCUGAuaGUGACg -3' miRNA: 3'- -UGCu-----GAGGUGGuuuAUGGACU--CGUUG- -5' |
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9430 | 5' | -50 | NC_002531.1 | + | 115781 | 0.69 | 0.952009 |
Target: 5'- -aGACUCCAgCCAGAUAauuucacccggUCUGAGUAGg -3' miRNA: 3'- ugCUGAGGU-GGUUUAU-----------GGACUCGUUg -5' |
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9430 | 5' | -50 | NC_002531.1 | + | 103087 | 0.7 | 0.9414 |
Target: 5'- gACGGCUUCAaugauuggugguUGCCUGAGUAACa -3' miRNA: 3'- -UGCUGAGGUgguuu-------AUGGACUCGUUG- -5' |
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9430 | 5' | -50 | NC_002531.1 | + | 81342 | 0.72 | 0.881094 |
Target: 5'- cACG-CUCUACCAgaagcAGUGCCUGAGaAACc -3' miRNA: 3'- -UGCuGAGGUGGU-----UUAUGGACUCgUUG- -5' |
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9430 | 5' | -50 | NC_002531.1 | + | 38087 | 1.11 | 0.006444 |
Target: 5'- uACGACUCCACCAAAUACCUGAGCAACa -3' miRNA: 3'- -UGCUGAGGUGGUUUAUGGACUCGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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