Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9431 | 5' | -55.4 | NC_002531.1 | + | 43311 | 0.66 | 0.849851 |
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Target: 5'- -aUCCUGCUauCCAaGCACCCCUa--- -3' miRNA: 3'- aaAGGACGGc-GGUaUGUGGGGAguuu -5' |
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| 9431 | 5' | -55.4 | NC_002531.1 | + | 55137 | 0.66 | 0.8241 |
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Target: 5'- --gCCaUGCUGCCAUGCcggguauagaggGCCCCaUCAAu -3' miRNA: 3'- aaaGG-ACGGCGGUAUG------------UGGGG-AGUUu -5' |
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| 9431 | 5' | -55.4 | NC_002531.1 | + | 38436 | 0.67 | 0.793806 |
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Target: 5'- gUUUCC-GCUGCUcgggcaaucggcguGUGCugCCCUCAAu -3' miRNA: 3'- -AAAGGaCGGCGG--------------UAUGugGGGAGUUu -5' |
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| 9431 | 5' | -55.4 | NC_002531.1 | + | 35379 | 0.68 | 0.747762 |
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Target: 5'- -----aGCCGCUAUcUACCCCUCAGc -3' miRNA: 3'- aaaggaCGGCGGUAuGUGGGGAGUUu -5' |
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| 9431 | 5' | -55.4 | NC_002531.1 | + | 37533 | 0.7 | 0.652625 |
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Target: 5'- --aCCUGCCccaGCCAUccccucaGCACCCC-CAAAa -3' miRNA: 3'- aaaGGACGG---CGGUA-------UGUGGGGaGUUU- -5' |
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| 9431 | 5' | -55.4 | NC_002531.1 | + | 90137 | 0.7 | 0.632326 |
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Target: 5'- --aUCUGCUagGCCAUGCACCCCa---- -3' miRNA: 3'- aaaGGACGG--CGGUAUGUGGGGaguuu -5' |
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| 9431 | 5' | -55.4 | NC_002531.1 | + | 39188 | 1.04 | 0.00436 |
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Target: 5'- cUUUCCUGCCGCCAUACACCCCUCAAAa -3' miRNA: 3'- -AAAGGACGGCGGUAUGUGGGGAGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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