Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9432 | 3' | -60.2 | NC_002531.1 | + | 63630 | 0.66 | 0.726613 |
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Target: 5'- -aGC-CCCCAGGgGCcaagauauuccaguGcCGUGCCUGAg -3' miRNA: 3'- ggUGcGGGGUCCgUG--------------CaGCACGGACU- -5' |
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| 9432 | 3' | -60.2 | NC_002531.1 | + | 62845 | 0.66 | 0.710147 |
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Target: 5'- gCACGCCCCccacugcGGCAgG-CGUGCUUu- -3' miRNA: 3'- gGUGCGGGGu------CCGUgCaGCACGGAcu -5' |
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| 9432 | 3' | -60.2 | NC_002531.1 | + | 19991 | 0.66 | 0.690543 |
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Target: 5'- gCACGauaCCCGauGGCACuauGUC-UGCCUGAa -3' miRNA: 3'- gGUGCg--GGGU--CCGUG---CAGcACGGACU- -5' |
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| 9432 | 3' | -60.2 | NC_002531.1 | + | 95313 | 0.68 | 0.61092 |
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Target: 5'- aCACaGCCCaUGGGCugGUUGaUGCCgGAc -3' miRNA: 3'- gGUG-CGGG-GUCCGugCAGC-ACGGaCU- -5' |
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| 9432 | 3' | -60.2 | NC_002531.1 | + | 37114 | 0.7 | 0.474688 |
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Target: 5'- aCCugGgCCCCAaaaucgcGGCAC-UCGUGgCUGAc -3' miRNA: 3'- -GGugC-GGGGU-------CCGUGcAGCACgGACU- -5' |
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| 9432 | 3' | -60.2 | NC_002531.1 | + | 46260 | 0.72 | 0.356877 |
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Target: 5'- gCCGCGUUCCAGG-GCGUCGaUGCCa-- -3' miRNA: 3'- -GGUGCGGGGUCCgUGCAGC-ACGGacu -5' |
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| 9432 | 3' | -60.2 | NC_002531.1 | + | 39842 | 0.77 | 0.181996 |
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Target: 5'- -aGCGCuUUCAGGCACGaCGUGCCUGGg -3' miRNA: 3'- ggUGCG-GGGUCCGUGCaGCACGGACU- -5' |
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| 9432 | 3' | -60.2 | NC_002531.1 | + | 40004 | 1.1 | 0.000905 |
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Target: 5'- cCCACGCCCCAGGCACGUCGUGCCUGAa -3' miRNA: 3'- -GGUGCGGGGUCCGUGCAGCACGGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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