Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9432 | 5' | -42.9 | NC_002531.1 | + | 109391 | 0.66 | 1 |
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Target: 5'- aGGUCuGUGACcaugaggcccGAgGAGC--AAGAGUCu -3' miRNA: 3'- -CCAGuUACUG----------UUgCUUGaaUUCUCAG- -5' |
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| 9432 | 5' | -42.9 | NC_002531.1 | + | 95616 | 0.66 | 1 |
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Target: 5'- gGGUCAGuUGGCGGaGGGCUcuGGGGUg -3' miRNA: 3'- -CCAGUU-ACUGUUgCUUGAauUCUCAg -5' |
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| 9432 | 5' | -42.9 | NC_002531.1 | + | 126023 | 0.66 | 1 |
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Target: 5'- --cCAGUGugGACGGGgUgugAAGAGUg -3' miRNA: 3'- ccaGUUACugUUGCUUgAa--UUCUCAg -5' |
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| 9432 | 5' | -42.9 | NC_002531.1 | + | 82050 | 0.67 | 0.999999 |
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Target: 5'- gGGUCGccUGACGGgGAGacacaggGAGAGUCu -3' miRNA: 3'- -CCAGUu-ACUGUUgCUUgaa----UUCUCAG- -5' |
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| 9432 | 5' | -42.9 | NC_002531.1 | + | 100482 | 0.67 | 0.999999 |
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Target: 5'- aGGUUggUGG-AGCGAGCagUGAGGGcCa -3' miRNA: 3'- -CCAGuuACUgUUGCUUGa-AUUCUCaG- -5' |
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| 9432 | 5' | -42.9 | NC_002531.1 | + | 39969 | 1.16 | 0.021053 |
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Target: 5'- aGGUCAAUGACAACGAACUUAAGAGUCg -3' miRNA: 3'- -CCAGUUACUGUUGCUUGAAUUCUCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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