Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9435 | 3' | -50.8 | NC_002531.1 | + | 130048 | 0.67 | 0.976711 |
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Target: 5'- ----cGUCAGGg---GGGUUGGGGGGg -3' miRNA: 3'- acauuCGGUCCaucaCCUAAUCCCUC- -5' |
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| 9435 | 3' | -50.8 | NC_002531.1 | + | 323 | 0.67 | 0.96801 |
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Target: 5'- --gGAGCCGuGG-AGUuaGGGUUAGGGAu -3' miRNA: 3'- acaUUCGGU-CCaUCA--CCUAAUCCCUc -5' |
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| 9435 | 3' | -50.8 | NC_002531.1 | + | 12608 | 0.68 | 0.957221 |
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Target: 5'- cGUAGGCUAGGUGcUGGA--GGGuGAa -3' miRNA: 3'- aCAUUCGGUCCAUcACCUaaUCC-CUc -5' |
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| 9435 | 3' | -50.8 | NC_002531.1 | + | 33730 | 0.68 | 0.957221 |
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Target: 5'- --gGAGUUGGGguugcaaggGGUGGGUUGGGGGa -3' miRNA: 3'- acaUUCGGUCCa--------UCACCUAAUCCCUc -5' |
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| 9435 | 3' | -50.8 | NC_002531.1 | + | 83088 | 0.68 | 0.95313 |
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Target: 5'- aGUcuGUgAGGUAGUGGAU-GGGGu- -3' miRNA: 3'- aCAuuCGgUCCAUCACCUAaUCCCuc -5' |
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| 9435 | 3' | -50.8 | NC_002531.1 | + | 107168 | 0.72 | 0.843786 |
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Target: 5'- aUGUccAGGCCauugauguuAGGUGGUGGAg-AGGGGGc -3' miRNA: 3'- -ACA--UUCGG---------UCCAUCACCUaaUCCCUC- -5' |
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| 9435 | 3' | -50.8 | NC_002531.1 | + | 49405 | 0.73 | 0.779351 |
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Target: 5'- cGUAGGCCGGGUcuggcagagAGUGGucgUAGaGGAa -3' miRNA: 3'- aCAUUCGGUCCA---------UCACCua-AUC-CCUc -5' |
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| 9435 | 3' | -50.8 | NC_002531.1 | + | 54731 | 0.74 | 0.707731 |
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Target: 5'- uUGUGGGaUCAGGgGGUGGGUagcUGGGGGGu -3' miRNA: 3'- -ACAUUC-GGUCCaUCACCUA---AUCCCUC- -5' |
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| 9435 | 3' | -50.8 | NC_002531.1 | + | 54928 | 1.09 | 0.006935 |
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Target: 5'- aUGUAAGCCAGGUAGUGGAUUAGGGAGc -3' miRNA: 3'- -ACAUUCGGUCCAUCACCUAAUCCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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