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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9436 | 5' | -58.5 | NC_002531.1 | + | 47102 | 0.66 | 0.744409 |
Target: 5'- -gUGAGGAGGUUcuaucuuaagagGAcuGAGGUGGUCUUg -3' miRNA: 3'- cuGCUCCUCCAGa-----------CU--CUCCACCGGAG- -5' |
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9436 | 5' | -58.5 | NC_002531.1 | + | 118443 | 0.69 | 0.573685 |
Target: 5'- aGACGAGG-GGcCUGAggaaccaGAGG-GGCCUg -3' miRNA: 3'- -CUGCUCCuCCaGACU-------CUCCaCCGGAg -5' |
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9436 | 5' | -58.5 | NC_002531.1 | + | 56928 | 1.1 | 0.001172 |
Target: 5'- aGACGAGGAGGUCUGAGAGGUGGCCUCa -3' miRNA: 3'- -CUGCUCCUCCAGACUCUCCACCGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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